FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1215479
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT273582.2507998904135738No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG249002.048575088504203No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT177431.459753726720083No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA172761.421332659799141No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG21710.1786127115318323No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT36750.0231.176598
GCAGGTC42100.0198.996282
CAGGTCA49550.0168.778883
AGTCTTT50550.0168.066079
GTCAATT51150.0162.057596
GCCATAC54150.0158.5271
AATTTCA55000.0150.713569
ATTAGGC54450.0148.714452
GGCAGGT57750.0147.623181
AGGCTGT57200.0140.275425
AGGTCAA67200.0124.449314
GGTCAAT67650.0123.403455
TCAATTT70250.0118.626267
ACCCCGC46950.0118.122058
TTAGGCT69650.0116.471663
CTAGTCT75000.0113.473187
TCTTACC49350.0112.975254
ATCTTAC49800.0112.250583
CTTACCC50150.0111.467175
CCATACT79100.0109.083252
Produced by FastQC (version 0.11.5)