FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444696

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444696
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1074317
Sequences flagged as poor quality0
Sequence length301
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT297132.765757220634133No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA251172.337950530430031No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT203431.893575173808103No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG196811.831954627917086No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG32970.3068926583122114No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT37250.0225.696698
AGTCTTT46550.0181.239539
AGGCTGT56900.0180.933825
ATTAGGC59900.0172.36452
GCCATAC50600.0167.31621
CAGGTCA41000.0163.323473
GCAGGTC41550.0160.806562
ATCTTAC40800.0156.532443
GTCAATT43000.0154.354986
TTAGGCT68850.0149.744143
CTTACCC43700.0146.482225
GCTGTGG71150.0144.488897
ACCCCGC46150.0138.38628
GGTGTCC555.921538E-8134.086151
TCTTACC48700.0131.442984
AATTTCA50450.0130.976539
GGCTGTG78950.0130.40076
TACCCCG49250.0129.67567
CTGTGGG80750.0127.6765758
CCCCGCC50900.0125.18229
Produced by FastQC (version 0.11.5)