FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1074317
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT276072.569725695488389No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA268212.496562932542257No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT200621.867419020642883No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG193511.8012374373671831No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG34100.31741096901566296No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT40350.0209.124888
AGGCTGT56750.0181.418735
ATTAGGC60250.0173.564742
AGTCTTT48900.0172.861779
GCCATAC51950.0163.519241
GCAGGTC39350.0163.048522
CAGGTCA40550.0159.678343
ATCTTAC41400.0156.399923
TTAGGCT67300.0153.848893
GTCAATT41550.0153.371876
GCTGTGG67900.0149.476157
AAGGTCC1050.0140.42406295
CTTACCC46100.0139.821235
AATTTCA47900.0132.737929
TAGGCTG78050.0132.658954
ACCCCGC48800.0131.196768
TCTTACC50000.0129.794134
TACCCCG49600.0129.08077
CTGTGGG78650.0128.857968
GGCTGTG79950.0127.3103266
Produced by FastQC (version 0.11.5)