FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339632
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG70472.0748928251754837No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT66881.9691901823149762No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT57081.6806425778489658No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA41511.222205210345315No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG3870.11394686013096528No Hit
AATTAGGCTGTGGGTGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATT3610.10629151552268337No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGC403.45608E-11221.25295
TAGTCTT9250.0212.081098
GAGGTCC150.0028488163196.66666295
GCAGGTC10900.0192.155962
CCGGTCC255.5530734E-5177.0295
GTCAATT12400.0165.342746
CAGGTCA13400.0156.305973
GGTGCCA200.0067356206147.51
AGTCTTT13450.0146.951689
AATTTCA14350.0145.958199
ATTAGGC13250.0141.377372
TCTCGCC1600.0138.28125295
GGCAGGT15250.0137.344251
GCCATAC14200.0136.073941
ACCCCGC14350.0130.540078
ATCTTAC14550.0129.759453
AGGCTGT14650.0127.86695
CCGCGCC352.1217437E-4126.428566295
TCTTACC15150.0124.620464
TCTCGTT609.9347744E-8122.91666295
Produced by FastQC (version 0.11.5)