FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339632
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG77472.28099825693692No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT59891.7633791868846278No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT51301.5104583784802375No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA43721.287275639515711No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG4160.12248551373251049No Hit
AATTAGGCTGTGGGTGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATT3520.10364158854289347No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT9900.0207.083348
GCAGGTC13250.0205.930852
CAGGTCA14900.0183.126433
GTCCATG351.0058466E-6168.561164
AATTTCA15950.0168.296979
GTCAATT16050.0167.248386
AAGGTCC451.7827915E-8163.90303295
GCCATAC12900.0158.924421
AGTCTTT13300.0154.144749
GGCAGGT18250.0149.511441
ACGGTCC301.14797534E-4147.51273295
AGGTCAA19450.0138.770464
GGTCAAT19850.0135.974095
ATTAGGC12500.0134.511812
ACCCCGC11600.0132.233348
TCTTACC12400.0126.081034
TCAATTT21400.0125.436297
ATCTTAC12850.0121.665743
AGGCTGT14700.0112.374115
TACCCCG13850.0110.751387
Produced by FastQC (version 0.11.5)