FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences562168
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA84811.5086237565994507No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT84391.5011526803375503No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT56561.0061049366025814No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG53550.9525622233922955No Hit
AATTAGGCTGTGGGTAGAAGTAGAGGTTAAGGAGGGTGATGGTGGCTATG9870.17557029215465839No Hit
AATTAGGCTGTGGGTGGGGATGATGAGGCTATTGTTTTTTGTGAATTCTT5640.10032588123123337No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGCA100.0014760017245.000023
CTGTCGG100.0014760017245.000028
TGTCGGT100.0014760017245.000029
TCGTACC204.7850193E-5183.750024
GGCCGTG150.0049662916163.333336
GCAGGGC150.0049662916163.333332
AGGCTGT21950.0154.03195
ATCTTAC14600.0149.349323
GTACCCC251.1644128E-4147.06
CGTACCC251.1644128E-4147.05
CTTACCC15650.0142.460075
ATTAGGC28600.0122.928322
GCTGTGG27050.0122.726437
GCTGGGG602.1027518E-9122.499997
TTAGGCT28300.0120.7685553
GGGTTAT14850.0118.78788245
TAGTCTT14650.0110.375438
TCAATGT354.4441244E-4105.07
TGAGCAG354.4441244E-4105.0245
AAAGCTT354.4441244E-4105.01
Produced by FastQC (version 0.11.5)