FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614572
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT105091.7099705160664658No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA100891.6416302727752No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT84051.3676184401502183No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG77681.2639690711584648No Hit
AATTAGGCTGTGGGTAGAAGTAGAGGTTAAGGAGGGTGATGGTGGCTATG6690.10885624467108818No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCC100.0014760349245.000026
CGTACCC150.0049664034163.333335
TAGTCTT16250.0153.784628
AGGCTGT27600.0147.79895
GCAGGTC17300.0135.245672
GTCAATT17550.0134.017096
CAGGTCA17800.0133.511233
ATTAGGC33100.0126.941092
AGTCTTT20200.0124.925749
ATCTTAC17400.0122.499993
TTAGGCT34200.0120.709063
GCTGTGG34100.0118.189157
CTTACCC19150.0112.584865
TAGGCTG40200.0103.302244
GCCATAC24100.0101.659751
CTGTGGG39800.0100.646998
TCAATTT24050.097.796257
GGCTGTG41900.097.649166
AATTTCA24100.097.593369
ACCCCGC22500.097.455558
Produced by FastQC (version 0.11.5)