FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614572
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA101901.6580644741380994No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT98161.5972091146358767No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG85041.3837272117831596No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT80571.3109936671374551No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACCCG204.7852343E-5183.750026
CGTACCT150.0049664034163.333335
TTCGGCC150.0049664034163.33333245
AGGCTGT28450.0154.57825
TCGTACC251.1644654E-4146.999984
TAGTCTT16550.0137.673728
ATTAGGC33700.0135.949552
CAGGTCA17500.0132.33
TTAGGCT34700.0131.678683
GTCAATT17550.0129.829066
GCAGGTC18700.0123.155082
GCTGTGG36050.0120.6310657
ATCTTAC16500.0115.8181843
AGTCTTT20550.0112.068139
GGCTGTA458.850828E-6108.888896
CTTACCC17750.0107.661975
AATTTCA21500.0107.116289
TAGGCTG43550.0105.76354
GCCATAC21900.0103.4817351
CTGTGGG41400.0102.6751258
Produced by FastQC (version 0.11.5)