FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1142457
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT382103.344546009171461No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT231542.0266845929431043No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG212221.8575753835811764No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA150211.3147978435949887No Hit
AATTAGGCTGTGGGTGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATT114220.999775046238064No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG60810.5322738623860679No Hit
GGCAGGTCAATTTCACTGGTAGTAATATAATTGTTGGGACGATTAGTTTT51060.446931481885095No Hit
AATTAGGCTGTGGGTGGTTGTGGGTATAATACTAAGTTGAGATGATATCA24690.2161131666224637No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT33100.0212.555548
ATCTTAC61100.0205.19173
GCGCGGG150.0028498904196.66214295
CTTACCC65200.0191.383645
ACCCCGC65250.0190.784888
AGGCTGT60200.0189.39355
TACCCCG67400.0184.6997
ATTAGGC63700.0180.1452
CCCCGCC69750.0178.687649
TCTTACC71850.0174.491464
AAATCTT73200.0171.273391
TTACCCC74100.0167.998846
AGTCTTT42100.0167.466459
GCTGTGG71800.0158.384257
TTAGGCT72700.0157.235033
GCCATAC45350.0155.139771
AGATCCC200.006738155147.49661295
CGCGTAA301.1491813E-4147.4966295
GCAGGTC61900.0144.160662
CAGGTCA64550.0138.699373
Produced by FastQC (version 0.11.5)