FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513090
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT72881.4204135726675633No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG61261.1939425831725428No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA60641.1818589331306397No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT54621.0645305891753882No Hit
AATTAGGCTGTGGGTAGAAGTAGAGGTTAAGGAGGGTGATGGTGGCTATG13040.2541464460426046No Hit
AATTAGGCTGTGGGTGGGGATGATGAGGCTATTGTTTTTTGTGAATTCTT6610.12882730125319145No Hit
AATTAGGCTGTGGGTGTGATAGGTGGCACGGAGAATTTTGGATTCTCAGG5880.11459977781675729No Hit
AATTAGGCTGTGGGTGGTGATTAGTCGGTTGTTGATGAGATATTTGGAGG5690.11089672377165799No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACCC700.0210.05
TCGTACC700.0210.04
GTACCCC900.0163.333346
AGGCTGT21050.0144.323045
ATTAGGC23600.0135.995762
ATCTTAC15650.0131.50163
CTTACCC16150.0127.4303365
TTAGGCT25350.0123.224853
GTCAATT13250.0121.11326
TAGTCTT12700.0116.71268
CAGGTCA14250.0116.052633
GCAGGTC14350.0115.2439042
GCTGTGG27400.0109.087597
ACCCCGC20800.0106.598568
GGGTTAT13250.0101.698105245
TACCCCG22750.098.07
CCCCGCC22700.097.136569
AGTCTTT15450.095.938519
TAGGCTG32450.095.5084844
AATTAGG35400.094.4703451
Produced by FastQC (version 0.11.5)