FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513090
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT68461.334268841723674No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG65461.2757995673273694No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA63201.2317527139488198No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT49550.9657175154456333No Hit
AATTAGGCTGTGGGTAGAAGTAGAGGTTAAGGAGGGTGATGGTGGCTATG8300.1617649924964431No Hit
AATTAGGCTGTAGGTAGAAGTAGAGGTTAAGGAGGGTGATGGTGGCTATG5800.11304059716618917No Hit
AATTAGGCTGTGGGTGTGATAGGTGGCACGGAGAATTTTGGATTCTCAGG5790.11284569958486815No Hit
AATTAGGCTGTGGGTGGTGATTAGTCGGTTGTTGATGAGATATTTGGAGG5440.1060242842386326No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACCC304.800313E-9204.166675
TCGTACC700.0175.04
TACCTCG452.8376235E-10163.333347
TACGACG150.004966166163.33333245
CGATCTA150.004966166163.333332
CGTACCT402.67828E-8153.1255
AGGCTGT21150.0145.957445
GGCTGTA950.0141.84216
ATCTTAC12500.0138.180013
GTACCCC455.410584E-8136.111116
GCAGGTC15200.0132.171052
CAGGTCA15750.0127.555563
TTAGGCT26750.0123.186923
GTACCTC501.0145595E-7122.499996
GTCAATT16000.0122.499996
ATTAGGC27950.0118.336312
CTTACCC14950.0116.3545155
GCTGTGG26750.0109.44867
TAGTCTT12550.0105.418338
AATTTCA18250.0104.7123269
Produced by FastQC (version 0.11.5)