FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005444712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349932
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT138623.9613410605489063No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT127333.63870694877862No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA96052.744818993404433No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG76582.1884251797492085No Hit
AATTAGGCTGTGGGTGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATT39251.1216464913183133No Hit
GGCAGGTCAATTTCACTGGTAGTAATATAATTGTTGGGACGATTAGTTTT16710.4775213470045609No Hit
AATTAGGCTGTGGGTGGTTGTGGGTATAATACTAAGTTGAGATGATATCA12110.3460672359201216No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG8200.23433124149834827No Hit
GGCAGGTCAATTTCACTGGATAAGTGGCGTTGGCTTGCCATGATTGTGAG6980.19946732508030132No Hit
AATTAGGCTGTGGGTGGTTGGTGTAAATGAGTGAGGCAGGAGTCCGAGGA4070.11630831132905821No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)