FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005444713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349932
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT128183.6629973823485704No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT119083.402946858246745No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA99002.829121086382497No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG79622.2752992009876203No Hit
AATTAGGCTGTGGGTGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATT37701.0773521712789913No Hit
GGCAGGTCAATTTCACTGGTAGTAATATAATTGTTGGGACGATTAGTTTT13230.3780734542711155No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG8850.25290627893419293No Hit
AATTAGGCTGTGGGTGGTTGTGGGTATAATACTAAGTTGAGATGATATCA8700.24861973183361338No Hit
GGCAGGTCAATTTCACTGGATAAGTGGCGTTGGCTTGCCATGATTGTGAG7270.2077546494747551No Hit
AATTAGGCTGTGGGTGGTTGGTGTAAATGAGTGAGGCAGGAGTCCGAGGA3910.11173599442177339No Hit
AATTAGGCTGTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCA3900.11145022461506807No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)