FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320659
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG72452.259409528502241No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT60061.8730177540627269No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA43931.369991174425168No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT36191.1286132620634381No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT9900.0213.055568
GCAGGTC12400.0206.97582
CAGGTCA13550.0190.498153
GTCAATT14550.0175.3786
AATTTCA15100.0168.990079
GCCATAC13100.0164.389311
ATTAGGC11500.0157.760882
AGTCTTT14050.0150.124549
GGCAGGT17100.0150.087721
AGGTCAA17200.0149.215124
GGTCAAT18050.0141.37125
TCAATTT19550.0131.278787
AGGCTGT14600.0123.2534265
CCATACT19750.0110.531652
CAATTTC23400.0110.309838
CTAGTCT19250.0109.5714347
ACCCCGC9950.0105.251258
ATACTAG20200.0105.148514
TTAGGCT17850.0102.464993
TCTTACC10450.0101.626794
Produced by FastQC (version 0.11.5)