FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1103527
Sequences flagged as poor quality0
Sequence length301
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT230232.086310529783141No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG189181.7143214438794883No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT175351.5889960100659068No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA164131.487322013870073No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG42930.38902537047122543No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT33650.0213.457768
GCAGGTC38500.0165.114272
AGTCTTT46000.0156.1499
CAGGTCA42100.0150.64493
GCCATAC48000.0149.335511
AGGCTGT45450.0147.654135
GCGCGCT200.0067387717147.49187295
ATATCGA401.9574545E-6147.49187295
ATTAGGC46250.0145.4192
GTCAATT45200.0139.660436
AATTTCA49800.0127.3524259
ATCTTAC39450.0125.620453
ACCCCGC41900.0118.627118
GGCAGGT54350.0118.047771
CTTACCC42400.0116.880345
TTAGGCT58150.0115.1527253
GCTGTGG58400.0114.407227
TCTTACC43700.0113.403364
AGGTCAA57750.0110.076184
GGTCAAT59300.0107.1989755
Produced by FastQC (version 0.11.5)