FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1103527
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT211701.918394384550627No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG185431.6803394932792763No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA171111.550573751253934No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT167431.5172261303982593No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG44450.40279938778117796No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT31750.0199.79668
GCAGGTC35900.0174.614032
CAGGTCA39150.0159.74913
ATTAGGC50800.0157.950062
GTCAATT41200.0151.814166
AGGCTGT51250.0151.684335
AGTCTTT42200.0149.971319
GCCATAC43850.0145.647461
AATTTCA46900.0134.942219
TTAGGCT59150.0134.910833
ATCTTAC40100.0132.054863
GCTGTGG63600.0120.6172947
TCTTACC44100.0120.0825354
GGCAGGT53450.0119.212191
ACCCCGC44400.0118.28528
CTTACCC44550.0117.214015
AGGTCAA53450.0116.739194
GGTCAAT54950.0114.089385
TACCCCG46550.0112.188147
CCGGTCC1200.0110.578295
Produced by FastQC (version 0.11.5)