FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1629816
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT547713.3605634010219556No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT473352.9043155791819446No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA376072.307438385682801No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG360402.211292563086876No Hit
AATTAGGCTGTGGGTGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATT132970.8158589681289177No Hit
GGCAGGTCAATTTCACTGGTAGTAATATAATTGTTGGGACGATTAGTTTT80890.49631369430659655No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG78600.48226302846456287No Hit
GGCAGGTCAATTTCAGTGGTAGTAATATAATTGTTGGGACGATTAGTTTT34040.20885793242918219No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT68500.0216.855678
ATCTTAC79700.0209.485213
AGGCTGT98650.0204.086465
CTTACCC84950.0197.065725
ATTAGGC103000.0196.31442
AGTCTTT78850.0188.577879
ACCCCGC91400.0183.18158
TTAGGCT110500.0182.862053
TCTTACC91650.0182.653784
GCCATAC83300.0177.746351
TACCCCG95950.0174.956137
GCTGTGG114350.0174.668437
AAATCTT100550.0166.026081
CCCCGCC101150.0165.816029
TTACCCC102250.0163.882896
AAGGTCC1650.0160.85475295
CAGGTCA95350.0157.158653
GCAGGTC96100.0156.08082
GTCAATT96250.0155.554986
GGCTGTG130100.0153.971766
Produced by FastQC (version 0.11.5)