FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005444720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366675
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG82622.2532215176927797No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT79322.1632235631008387No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT77692.11877002795391No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA49711.355696461444058No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG8060.2198131860639531No Hit
AATTAGGCTGTGGGTGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATT7360.20072271084748075No Hit
AATTAGGCTGTGGGTAGAAGTAGAGGTTAAGGAGGGTGATGGTGGCTATG4160.11345196700074998No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)