FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences849074
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT223042.6268617340773592No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA206012.426290288007877No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT160711.8927678859557588No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG117421.3829183322066156No Hit
AATTAGGCTGTGGGTGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATT29330.3454351446399254No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG22840.2689989329551959No Hit
GGCAGGTCAATTTCACTGGTAGTAATATAATTGTTGGGACGATTAGTTTT13500.15899674233341263No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT34450.0203.367588
AGGCTGT44450.0180.843175
ATTAGGC45600.0175.958972
AGTCTTT41250.0170.915459
GCCATAC43100.0163.236951
ATCTTAC31500.0162.946873
CTTACCC32050.0160.15065
TTAGGCT52400.0153.406083
GCTGTGG52900.0152.234947
ACCCCGC35700.0143.363518
TCTTACC37550.0136.693134
CAGGTCA30400.0136.335863
GCAGGTC30550.0135.183652
TACCCCG38800.0132.289357
CCCCGCC38900.0131.57019
GGCTGTG63450.0127.387356
GTCAATT32600.0126.6828466
TAGGCTG63900.0126.02864
CTGTGGG64550.0124.759548
TCTCGTT950.0124.20634295
Produced by FastQC (version 0.11.5)