FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences849074
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA211972.496484405363961No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT210382.4777581223780256No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT157721.8575530519130252No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG114201.3449946647759794No Hit
AATTAGGCTGTGGGTGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATT24120.28407417963569725No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG22690.26723230248482466No Hit
GGCAGGTCAATTTCACTGGTAGTAATATAATTGTTGGGACGATTAGTTTT10210.12024864734993651No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCTGT46550.0191.081595
TAGTCTT32350.0190.144178
ATTAGGC50200.0178.353092
TTAGGCT53950.0165.955983
AGTCTTT37000.0165.8499
ATCTTAC29300.0163.61063
GCTGTGG55800.0158.886547
GCCATAC39150.0157.080831
CTTACCC31200.0150.819435
GCAGGTC30200.0144.082342
TAGGCTG62650.0143.154084
ACCCCGC33650.0140.27698
CTGTGGG63000.0140.25158
GGCTGTG64350.0138.226096
CAGGTCA32100.0135.554113
GTCAATT32900.0134.059176
TACCCCG35750.0132.457287
TCTTACC36250.0131.843374
CCCCGCC37950.0125.548649
AATTAGG71550.0125.11171
Produced by FastQC (version 0.11.5)