FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005445146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005445146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37784976
Sequences flagged as poor quality0
Sequence length85
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3668650.9709282334862407No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3324470.8798391191250193No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1350680.35746482940732843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG582700.047.340461
TGGGGGG879100.036.0523831
TATGCCG65700.024.34808346-47
AATCGCG25400.024.25864638-39
TCGTATG60300.024.10483742-43
CGCGTAT15750.023.19716542-43
CGTATGC74650.021.53481544-45
CTCGTAT81100.017.92258642-43
ATCGCGT25950.017.7322240-41
CGTATCG68000.016.264513
GTATCGA46400.016.0039674
GTATGCC106050.015.88491644-45
TATCGAA54350.014.7533295
TGAATCG55650.014.37262636-37
CCGTATC84800.014.207162
TTGAATC217150.013.3691536-37
CCCGTAT69600.013.167831
AATCTCG150750.013.02191238-39
GCGTATG31300.012.3036542-43
ATCGAAG112300.012.2052676
Produced by FastQC (version 0.11.5)