FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005445147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005445147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38782908
Sequences flagged as poor quality0
Sequence length85
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT21431465.526006456246138TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG633010.16321880762525595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA2871500.064.946449
AGAGCAC2912600.064.090928
AAGAGCA2977800.062.8136527
CGGAAGA2984900.062.5788354
TCGGAAG2997700.062.0917133
GATCGGA2997850.060.838281
ATCGGAA3088150.060.301432
GAAGAGC3130250.059.8335276
GGAAGAG3331050.056.2899975
TCGTATG2354900.038.7662844-45
TATGCCG2358250.038.63667348-49
CGTATGC2377850.038.3904646-47
CTCGTAT2348800.038.24473644-45
TATCTCG2347500.038.22805440-41
GTATGCC2396500.038.05461546-47
ACGAGTG2430850.037.89222732-33
GCCGTCT2395300.037.8881650-51
CGAGTGG2429150.037.865932-33
GATATCT2377600.037.77981638-39
GTCACGA2446900.037.7445728-29
Produced by FastQC (version 0.11.5)