FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005445157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005445157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66773554
Sequences flagged as poor quality0
Sequence length85
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7045351.0551108302547443No Hit
TTTTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5116660.766270430955345No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4773610.7148953012146096No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC4320360.6470166317641263TruSeq Adapter, Index 8 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3064090.4588777766718842No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA1658950.24844416698263505No Hit
CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT1466580.21963485723704326No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATGC1375260.20595878422166955TruSeq Adapter, Index 8 (98% over 50bp)
GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG1218570.18249290729680198No Hit
GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA1030980.15439944981811213No Hit
CTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGGTGATTGT947810.14194391989379507No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTTTGC830190.12432916181157588TruSeq Adapter, Index 8 (98% over 50bp)
GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA771610.11555622754481512No Hit
GCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAG765850.11469361058720942No Hit
CCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAAT739450.11073994953151663No Hit
AGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAG737860.11050183130884422No Hit
TGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAG698580.10461926288961645No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA694580.1040202233357236No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)