FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005445177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005445177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24678063
Sequences flagged as poor quality0
Sequence length85
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7311502.9627527897955366No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7013422.8419653519808263No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1998160.8096907767842233No Hit
TTTTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1821860.7382508100412905No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1794560.7271883534781478TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG1091600.053.0828551
TTTTGGG350250.047.3310172
TTTGGGG406250.041.798093
TCGTATG205700.037.38745542-43
TTTTTGG433050.035.917321
TATGCCG215750.035.71828546-47
CTCGTAT209700.035.28040742-43
GAGCACA445550.035.140879
AGAGCAC454100.034.5575078
AAGAGCA467950.033.585217
CGTATGC233000.033.10814744-45
TTGGGGG539400.032.4903764
GTATGCC241000.032.03370744-45
GCCGTCT250500.030.72346948-49
CGGAAGA516250.030.083594
GATCGGA511300.029.7250751
TCGGAAG523100.029.622113
CTTGTAA260850.029.60761534-35
ATGCCGT269500.029.04885746-47
TAATCTC260150.028.4861738-39
Produced by FastQC (version 0.11.5)