FastQCFastQC Report
Thu 12 May 2022
EGAF00005445182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005445182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53665689
Sequences flagged as poor quality0
Sequence length85
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT2377320.4429869520542259No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA2371270.44185960232430815No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC2059040.3836790393206356TruSeq Adapter, Index 12 (100% over 50bp)
CTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGGTGATTGT1719100.32033502821514137No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1698790.31655048722098766No Hit
GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG1609010.29982098990660494No Hit
AGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAG1352800.2520791263855757No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGC1294380.24119321378693193TruSeq Adapter, Index 12 (98% over 50bp)
CCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAAT1249410.23281355802587383No Hit
TGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAG1227550.22874019189430328No Hit
CCGCTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAA1197490.22313884761639788No Hit
GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA1179680.21982015361807802No Hit
CGCTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAAT1178860.21966735580344457No Hit
CGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGC1135310.21155230113602008No Hit
GCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAG1122360.20913921369760108No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA1108330.2065248803569819No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA977310.1821107709993251No Hit
CTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTT885960.16508872177155873No Hit
GCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAG882260.16439926821772474No Hit
CTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATAT852160.15879047038788602No Hit
CTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGA833020.1552239457877826No Hit
GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA831060.15485872174304888No Hit
GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAA797770.14865550314652626No Hit
GGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATG719630.1340949894447456No Hit
CTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTT719460.13406331184902892No Hit
GAGCTTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATATATCCCATTT712340.1327365796048943No Hit
CCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGT709270.13216451949401042No Hit
CTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAGGTG700300.1304930604729588No Hit
GGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATA691860.12892036101502397No Hit
ATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATC680540.1268110058178886No Hit
GGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGT635410.11840153584909718No Hit
GGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGG632210.11780525169442994No Hit
CTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTCAC596180.11109146479047348No Hit
CACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGG594500.11077841560927318No Hit
GTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACC588980.1097498254424722No Hit
GGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGG586470.10928211505865507No Hit
GTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGT580670.1082013500283207No Hit
CCAGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGC557870.10395282542631662No Hit
AAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAA550020.10249006585939854No Hit
TGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTATA546230.10178384181371453No Hit
TCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGT540590.10073289099111352No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG539490.10052791831294666No Hit
GTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTA539490.10052791831294666No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)