FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005445189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005445189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32970764
Sequences flagged as poor quality0
Sequence length85
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4746461.4395966074671487No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4586451.3910657332659928No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4157591.2609929208798438No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC3382661.0259574209442037TruSeq Adapter, Index 3 (100% over 50bp)
TTTTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3355201.0176288301963523No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA631620.19156971916089055No Hit
CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT549510.1666658376493793No Hit
CTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGGTGATTGT469770.1424807747858072No Hit
ATTTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG467830.14189237471112287No Hit
GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG443280.13444638407529774No Hit
GCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAG374020.11343989481105139No Hit
GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA355130.10771057655806823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGGG626100.054.386882
TTTGGGG717650.048.973073
GAGCACA833500.048.8033839
AGAGCAC871100.046.8235138
AAGAGCA912000.044.9011347
TTTTTGG660250.044.06951
TTGGGGG841050.044.036734
CGGAAGA988400.041.1721884
TCGGAAG1029650.039.5269973
ATCGGAA1065300.038.248962
TCGTATG487200.038.02675242-43
GAAGAGC1095100.037.4635286
TATGCCG508000.037.0468846-47
GATCGGA1021150.036.998011
CTCGTAT444100.036.5893342-43
GGAAGAG1164500.035.2104875
CGTATGC542250.034.9810444-45
ACTTAGG571350.034.8913332-33
GTATGCC550500.034.1841444-45
TTAGGCA594700.032.79184334-35
Produced by FastQC (version 0.11.5)