FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005445197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005445197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33553702
Sequences flagged as poor quality0
Sequence length85
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT8895342.651075580274272TruSeq Adapter, Index 20 (97% over 44bp)
TTTTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4079981.2159552469053936No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3630361.0819551297201124No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2471280.7365148560954615No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1475380.43970707017663807No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATATCGTAT588360.17534875883442014TruSeq Adapter, Index 20 (97% over 43bp)
ATTTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG472350.14077433244176754No Hit
CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT370300.11036040076889281No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1654950.060.9550869
AGAGCAC1670200.060.485578
AAGAGCA1736600.058.2592967
TTTTGGG690700.057.2591172
CGGAAGA1859300.054.40124
ATCGGAA1870600.054.1892742
GAAGAGC1878050.053.9126746
TCGGAAG1898850.053.3827553
GATCGGA1845650.053.057341
TTTGGGG788900.051.4180763
GGAAGAG1995200.050.792675
TTTTTGG721300.048.5440141
TTGGGGG936200.045.0829054
TCGTATG1196250.038.86575744-45
TATGCCG1224350.038.19114748-49
CTCGTAT1102800.038.14386444-45
GTCACGT1311000.037.51467528-29
CGTATGC1262300.037.458346-47
TATCTCG1103600.037.26531640-41
GTATGCC1269750.036.91032446-47
Produced by FastQC (version 0.11.5)