FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005445198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005445198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35487440
Sequences flagged as poor quality0
Sequence length85
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT3350340.9440917688060904TruSeq Adapter, Index 22 (97% over 40bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3277830.9236591875886229No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG383650.10810867168778587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA793900.045.3328939
AGAGCAC806300.044.684848
CGGAAGA829850.043.273824
TCGGAAG826700.043.242173
AAGAGCA850450.042.4578637
GATCGGA843200.041.665461
ATCGGAA859300.041.5637632
AGGGGGG1451600.038.28091
CGTACGT463900.037.952134-35
TCGTATG367200.037.50884644-45
TACGTAA471950.037.43445636-37
ACGTAAT476650.036.97831736-37
GTACGTA480650.036.88017734-35
CACCGTA483850.036.63368630-31
TATGCCG366800.036.32750348-49
GAAGAGC999250.036.2976576
CGTAATC462800.035.7150738-39
CTCGTAT395500.035.2593444-45
CCGTACG502000.035.21731632-33
ACCGTAC507400.034.9865432-33
Produced by FastQC (version 0.11.5)