FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005445199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005445199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34259520
Sequences flagged as poor quality0
Sequence length85
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT2959320.863794939333651TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG347100.037.3086944-45
TATGCCG359400.036.13625348-49
CTCGTAT359100.035.67146744-45
GAGCACA768900.034.872599
CGTATGC377100.034.5029746-47
AGAGCAC780950.034.26378
TATCTCG379550.033.76511440-41
GTATGCC393300.032.9964346-47
GCCGTCT407450.031.66148650-51
AAGAGCA862900.031.2203277
ATGCCGT439650.029.56271248-49
TTATCTC434400.029.49717740-41
CGGAAGA903500.029.4037694
TCGGAAG932050.028.3801363
CTTTATC454200.028.22869738-39
TGCCGTC464800.028.13305750-51
ATCTCGT480400.027.00982742-43
GAAGAGC1004350.026.9197736
ACATTCC492500.026.81942732-33
CCTTTAT485200.026.81182136-37
Produced by FastQC (version 0.11.5)