FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005445200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005445200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32407938
Sequences flagged as poor quality0
Sequence length85
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4182541.29059121256033No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4094701.2634867420444955No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2789120.8606286521530621No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT2627000.8106038711873615TruSeq Adapter, Index 18 (97% over 40bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1185000.3656511562074699No Hit
ATTTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG420000.129597878149483No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA366160.11298466443622546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGGG696300.058.32962
TTTGGGG808200.051.7341423
TTTTTGG748400.048.558531
TTGGGGG979350.044.701094
GAGCACA724850.041.8471539
AGAGCAC746500.040.664818
AAGAGCA799300.038.2355237
TCGTATG364350.037.9080944-45
TATGCCG381500.036.58034548-49
CTCGTAT357150.035.3929344-45
CGTATGC405350.034.83305446-47
GTCCGCA413750.034.33057434-35
TCACGTC428700.034.11731730-31
GTCACGT430900.034.05330328-29
CGGAAGA895800.034.0381364
GTATGCC419300.033.35857446-47
GAAGAGC920950.033.2115756
CGTCCGC434650.033.1694232-33
AGTCACG451450.033.0937728-29
CGCACAT395900.032.9211336-37
Produced by FastQC (version 0.11.5)