FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005445203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005445203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37060000
Sequences flagged as poor quality0
Sequence length85
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13072573.5274069077172157No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT8810022.377231516459795TruSeq Adapter, Index 7 (97% over 36bp)
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA1124920.30354020507285484No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG785760.2120237452779277No Hit
CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT666040.17971937398812735No Hit
GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG549540.1482838640043173No Hit
GCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAG439100.11848354020507286No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG431860.11652995143011333No Hit
AGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAG420090.11335402050728549No Hit
GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAA390720.10542903399892067No Hit
CCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAAT386430.10427145169994603No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)