FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005445216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005445216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32467229
Sequences flagged as poor quality0
Sequence length85
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5410491.6664464959421081No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4973211.5317629970823812No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC4136431.2740323481255513TruSeq Adapter, Index 12 (100% over 50bp)
TTTTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4056381.249376717674305No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4003321.233034084922985No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATATCGTATGC485070.14940295643955326TruSeq Adapter, Index 12 (98% over 50bp)
ATTTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG432640.13325436550190348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGGG658550.057.889052
TTTGGGG781450.050.407193
TTTTTGG705600.048.2453731
GAGCACA1067400.046.0270089
AGAGCAC1096500.044.8699238
TTGGGGG947500.043.54474
AAGAGCA1151650.042.8037037
GAAGAGC1290500.038.2147066
TCGTATG603650.038.20406742-43
CGGAAGA1290900.038.0008664
TATGCCG608950.037.6714446-47
CGTATGC635750.036.5039244-45
CTCGTAT526900.036.391742-43
TCGGAAG1352650.036.2809683
ATCGGAA1358400.036.1128542
GTATGCC649500.035.5091444-45
GCCGTCT652600.034.3041348-49
GATCGGA1345050.034.046941
GGAAGAG1454600.033.8899045
CTTGTAA690750.033.68208734-35
Produced by FastQC (version 0.11.5)