FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005445222

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005445222
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20547161
Sequences flagged as poor quality0
Sequence length85
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5829902.837326285611915No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5111902.4878862826840162No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1387700.6753731087229033No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC921810.4486313218648552TruSeq Adapter, Index 9 (100% over 50bp)
TTTTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG685490.33361786574797364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG819250.056.8034551
TCGTATG113400.036.2950242-43
TATGCCG116600.035.28160546-47
CGTATGC127000.032.45484544-45
CTCGTAT123400.032.3294742-43
GTATGCC140350.029.48033744-45
TTTTGGG218900.029.1594982
GTCACGA143150.029.0710328-29
ACGATCA145550.028.7700432-33
AGTCACG151150.028.02892128-29
GCCGTCT149000.027.76842148-49
CACGATC151250.027.56828130-31
TTTGGGG248700.026.2370833
ATGCCGT160800.026.05027246-47
TCACGAT162650.025.97607630-31
CGATCAG170200.024.51044532-33
GATCTCG164950.024.2944138-39
TGCCGTC174850.024.14879448-49
GAGCACA350300.024.0959829
CCGTCTT173800.023.82879350-51
Produced by FastQC (version 0.11.5)