FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005445231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005445231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34111244
Sequences flagged as poor quality0
Sequence length85
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16257984.76616449403018No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11650933.415568778435638No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3706721.0866563529609181No Hit
TTTTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2173240.6371037069184577No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC1550900.45465946653836486TruSeq Adapter, Index 10 (100% over 50bp)
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA391280.11470704498493223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2050300.063.617281
TTTTGGG431000.046.2620932
TTTGGGG538350.037.8148963
TCGTATG179700.036.6092842-43
TTTTTGG524650.035.2266851
CTCGTAT192200.033.55014842-43
TATGCCG200100.032.92567446-47
TTGGGGG677800.030.902964
ACTAGCT214900.030.75368132-33
GCTTATC210750.030.49658636-37
CACTAGC226300.029.41368730-31
CGTATGC231450.028.43203744-45
TATCTCG227200.028.40905838-39
TCACTAG237300.027.9087230-31
TAGCTTA243850.027.20814734-35
GTATGCC245050.026.99110444-45
CTAGCTT248750.026.783132-33
GCCGTCT255550.025.90488248-49
CCGTCTT258300.025.41498250-51
TGGGGGG1226100.025.1172121
Produced by FastQC (version 0.11.5)