FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005445233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005445233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28823105
Sequences flagged as poor quality0
Sequence length85
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9566133.3189102978322427No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6734282.3364172596949566No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC5627211.9523260939444242TruSeq Adapter, Index 5 (100% over 50bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2048900.7108533240953742No Hit
TTTTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1033210.3584658904722444No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA448480.1555973931330438No Hit
CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT418570.1452203015601546No Hit
GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG315890.10959610354262667No Hit
CTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGGTGATTGT302110.10481521681997828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA937800.053.4054079
AGAGCAC955350.052.4532788
AAGAGCA993350.050.4941647
CGGAAGA1031750.048.2780534
TCGGAAG1054150.047.200133
ATCGGAA1073500.046.3090252
GATCGGA1060950.045.9011761
AGGGGGG1723950.044.2093281
GAAGAGC1143500.044.00566
GGAAGAG1232250.040.906725
TCGTATG637650.038.68185842-43
TATGCCG646500.038.35007546-47
CTCGTAT630500.037.72973642-43
CGTATGC678550.036.5565944-45
GTATGCC692600.035.74371744-45
GCCGTCT696400.035.44316548-49
CCGTCTT708300.034.93703550-51
AGTGATC674650.034.84230436-37
ATGCCGT723200.034.35653746-47
GATCTCG684650.034.1705738-39
Produced by FastQC (version 0.11.5)