FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005445248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005445248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37675033
Sequences flagged as poor quality0
Sequence length85
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT7357291.9528290791410854TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1345150.049.461629
AGAGCAC1378850.048.286878
AAGAGCA1463100.045.5521937
CGGAAGA1485750.044.7043344
TCGGAAG1510850.043.778623
GATCGGA1538650.041.55211
GAAGAGC1617350.041.351176
ATCGGAA1599800.041.3382452
TCGTATG844550.038.0399644-45
TATGCCG853850.037.51923448-49
CTCGTAT846150.037.3308344-45
CGTATGC874200.036.7520246-47
TATCTCG866900.036.56714240-41
GGAAGAG1835750.036.4914255
GTATGCC888200.036.09490646-47
GATATAT906050.035.71512636-37
ATATCTC893150.035.4592540-41
GCCGTCT900550.035.44858650-51
TATATCT896700.035.3386938-39
ACTGATA925600.035.00131234-35
Produced by FastQC (version 0.11.5)