FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005445251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005445251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37898419
Sequences flagged as poor quality0
Sequence length85
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3548610.9363477669081657No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA1440670.3801398681037328No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC1360510.35898858999896543TruSeq Adapter, Index 1 (100% over 50bp)
GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG1028250.271317386617104No Hit
GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA888960.23456387455107297No Hit
CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT804920.2123888070370429No Hit
AGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAG790150.2084915468373496No Hit
GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA638270.168415996456211No Hit
GCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAG637660.16825503987382692No Hit
GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAA558940.1474837248487859No Hit
TGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAG458220.1209074183279255No Hit
GGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGG457980.1208440911479711No Hit
GCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAG457440.12070160499307372No Hit
CTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATAT440200.1161526025663498No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA410960.10843724114190621No Hit
GGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGA406320.10721291566278793No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG404800.10681184352307677No Hit
CCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAAT393890.10393309546764998No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)