FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005446984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005446984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65168186
Sequences flagged as poor quality0
Sequence length31
%GC42

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAACCCC700120.10743278936749905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAC144250.024.7930721
TGCGCCA119200.024.7370381
GGGCGCG171550.024.6811083
AAACCCC1751900.024.6760125
AGGGCGC172100.024.6680582
CCCGAGC224500.024.5511552
CCGAAAC129350.024.5385426
TAGGGCC121600.024.518544
CCCGGCC246250.024.4913238
TAGCCCA327950.024.4718913
CCCGGCG72000.024.4639552
GGGTAAG143050.024.4599114
CCGCGGA95950.024.441962
GCCCGGC247900.024.4140267
CTTAATG318800.024.4135761
CGTTAAG250200.024.3900491
CCCGCGG96400.024.3871441
AAAACCC1765500.024.37987724
CAAATCC208050.024.3768423
GCCGACC194300.024.3662091
Produced by FastQC (version 0.11.5)