FastQCFastQC Report
Thu 12 May 2022
EGAF00005447004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005447004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97631536
Sequences flagged as poor quality0
Sequence length31
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCATGCCTCGGAAGAGCACACGTCTGAAC2076080.21264440620907574Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TCCGAATTCAAAAAAAAAAAAAAAAAAAAAC1018620.10433309171741392No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)