FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005447008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005447008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76522228
Sequences flagged as poor quality0
Sequence length31
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGCG166850.024.7710532
GGGCCAT349400.024.6813052
GAGGCGA305850.024.6682625
AAACCCC1169100.024.58698825
GCCGATG371750.024.5662123
ACCCGGC168150.024.5351161
GGGCGAA391050.024.5313458
CTGGAGC232350.024.515931
GTGCCCG281250.024.50421
GGCCCGT325650.024.5005326
CAGGCCC327700.024.4579144
CCCGGTC143000.024.45604110
GACGCCA306750.024.45602410
TGGGCGA393450.024.4548237
CAAATCC214150.024.44883
AGCCGAC68350.024.4439031
TGCGCCA95200.024.4199681
GCGGCAC39700.024.4125881
CTTATAC410900.024.407063
CGCTTTG514650.024.4034355
Produced by FastQC (version 0.11.5)