FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005466243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005466243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68794092
Sequences flagged as poor quality0
Sequence length5-100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG140100.015.9743113
GTATCGA143350.014.6363864
TATCGAA144850.014.6130195
CCGTATC171050.014.4740632
CCCGTAT168150.014.25318051
GCCGTAT80150.012.3332591
GTCCGTT79200.012.2457031
GTCCGAT65150.012.2384721
CGTCGGA173150.011.95768294
TTAGACG106800.011.917768
TCAATCG139800.011.7058558
GTCCTAT137750.011.4411751
CTACCTA177550.011.00667894
GTCGCAT52900.010.9297831
CAATCGG148050.010.8331139
GTCGGAT75000.010.7554941
GTCCATT343350.010.6197741
CGAATGG194800.010.47953594
ATCGAAG235350.010.0437616
TACAACG151400.09.7311577
Produced by FastQC (version 0.11.5)