FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005466334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005466334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences611741
Sequences flagged as poor quality0
Sequence length251
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA22720.3713990070961404No Hit
GGTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA18360.3001270145371979No Hit
CGTATGAGATAAGCCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG17730.2898285385481764No Hit
GGTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGTGTCTCCAGAGCA17700.2893381349296516No Hit
GGCCTGCGATGTTGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA16260.2657987612404596No Hit
AGCCTGCGATGTTGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA15630.2555002852514381No Hit
TGCCTGCGATGTTGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA15430.2522309277946059No Hit
CGTATGAGATAAGCCCAGGGTTTTCCCAGTCACGACAGGTCGTTTTTCTT13300.21741227087934273No Hit
GGTATGAGATAAGCCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG12950.21169089532988633No Hit
GGTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG12060.19714225464698296No Hit
CTTATGAGATAAGCCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG11570.18913232887774403No Hit
TATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT11450.1871707144036447No Hit
AGTATGAGATGCACCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG11190.1829205497097628No Hit
GGTATGAGATGCACCCAGGGTTTTCCCAGTCACGACAAGACTGAAGGTCA11080.18112240310850508No Hit
CGTATGAGATGCACCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG11010.1799781279986138No Hit
CCTATGAGATAAGCCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG10760.1758914311775735No Hit
AGTATGAGATGCACCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA10530.17213167010221647No Hit
AGTATGAGATGCACCCAGGGTTTTCCCAGTCACGACAAGACTGAAGGTCA10500.17164126648369163No Hit
CGCCTGCGATGTTGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA10440.17066045924664197No Hit
GGTATGAGATGCACCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG10430.17049699137380034No Hit
AGTATGAGATGCACCCAGGGTTTTCCCAGTCACGACGAGGCTGAATTTAT10430.17049699137380034No Hit
CATATGAGATAAGCCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG10360.16935271626390908No Hit
TCTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT10340.16902578051822584No Hit
AACCTGCGATGTTGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA10330.16886231264538423No Hit
ATCCTGCGATGTTGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA10270.16788150540833457No Hit
CATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT10010.16363134071445268No Hit
TTCCTGCGACAACTCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT9950.16265053347740302No Hit
GACCTGCGATGTTGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA9940.16248706560456141No Hit
TTTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT9890.16166972624035333No Hit
GGTATGAGATGCACCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA9870.16134279049467012No Hit
CGTATGAGATGCACCCAGGGTTTTCCCAGTCACGACAAGACTGAAGGTCA9780.15987157963909562No Hit
TACCTGCGATGTTGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA9780.15987157963909562No Hit
TTCCTGCGATGTTGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA9700.15856383665636276No Hit
GATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT9650.1577464972921547No Hit
GGTATGAGATGCACCCAGGGTTTTCCCAGTCACGACGAGGCTGAATTTAT9650.1577464972921547No Hit
CGTATGAGATGCACCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA9640.15758302941931307No Hit
TGTATGAGATAAGCCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG9640.15758302941931307No Hit
GGTATGAGATAAGCCCAGGGTTTTCCCAGTCACGACAGGTCGTTTTTCTT9460.15464060770816407No Hit
TACCTGCGACAACTCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT9460.15464060770816407No Hit
CCTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT9360.15300592897974796No Hit
TATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACTTAAGAGTCACGCT9300.15202512174269828No Hit
GTCCTGCGATGTTGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA9300.15202512174269828No Hit
GTTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA9230.15088084663280704No Hit
GCTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT9160.14973657152291575No Hit
CGTATGAGATGCACCCAGGGTTTTCCCAGTCACGACGAGGCTGAATTTAT9070.14826536066734125No Hit
AGTATGAGATAAGCCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG9010.14728455343029156No Hit
AACCTGCGACAACTCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT8860.1448325353376674No Hit
ATCCTGCGAAGGAGCCAGGGTTTTCCCAGTCACGACTTAAGAGTCACGCT8820.14417866384630096No Hit
TGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT8790.14368826022777614No Hit
GTTATGAGATAAGCCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG8760.1431978566092513No Hit
ATCCTGCGACAACTCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT8740.1428709208635681No Hit
GTTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA8710.14238051724504325No Hit
TTCCTGCGAAGGAGCCAGGGTTTTCCCAGTCACGACTTAAGAGTCACGCT8700.14221704937220164No Hit
TGTATGAGATGCACCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG8670.1417266457536768No Hit
AACCTGCGAAGGAGCCAGGGTTTTCCCAGTCACGACTTAAGAGTCACGCT8660.1415631778808352No Hit
GCTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA8580.1402554348981023No Hit
GGCCTGCGATGTTGCCAGGGTTTTCCCAGTCACGACCCTTCAATCTGAGG8510.139111159788211No Hit
GTTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT8500.1389476919153694No Hit
ATCCTGCGACGAGGCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT8470.1384572882968446No Hit
TGTATGAGATGCACCCAGGGTTTTCCCAGTCACGACAAGACTGAAGGTCA8470.1384572882968446No Hit
TCTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACTTAAGAGTCACGCT8450.13813035255116135No Hit
TATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT8440.13796688467831975No Hit
CATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACTTAAGAGTCACGCT8440.13796688467831975No Hit
GCTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA8370.13682260956842845No Hit
CTTATGAGATAAGCCCAGGGTTTTCCCAGTCACGACAGGTCGTTTTTCTT8220.13437059147580432No Hit
CCTATGAGATAAGCCCAGGGTTTTCCCAGTCACGACAGGTCGTTTTTCTT8140.13306284849307143No Hit
GACCTGCGACGAGGCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT8100.13240897700170498No Hit
CTTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT8040.1314281697646553No Hit
GATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACTTAAGAGTCACGCT8020.13110123401897208No Hit
TTCCTGCGACGAGGCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT8020.13110123401897208No Hit
TACCTGCGACGAGGCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT7940.1297934910362392No Hit
TGTATGAGATGCACCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA7940.1297934910362392No Hit
TGTATGAGATGCACCCAGGGTTTTCCCAGTCACGACGAGGCTGAATTTAT7930.12963002316339758No Hit
TCCCTGCGACAACTCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT7930.12963002316339758No Hit
TACCTGCGAAGGAGCCAGGGTTTTCCCAGTCACGACTTAAGAGTCACGCT7880.12881268379918953No Hit
TTTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACTTAAGAGTCACGCT7880.12881268379918953No Hit
CGTATGAGATAAGCCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT7840.12815881230782308No Hit
GATATGAGATAAGCCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG7810.12766840868929824No Hit
AACCTGCGACGAGGCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT7790.12734147294361503No Hit
CGTATGAGATGCACCCAGGGTTTTCCCAGTCACGACCCTTCAATCTGAGG7780.12717800507077343No Hit
TGCCTGCGATGTTGCCAGGGTTTTCCCAGTCACGACCCTTCAATCTGAGG7690.12570679421519892No Hit
AATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT7660.12521639059667408No Hit
CGTATGAGATAAGCCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT7630.12472598697814924No Hit
CTCCTGCGACAACTCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT7610.12439905123246603No Hit
GGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT7580.12390864761394119No Hit
TCTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT7560.12358171186825798No Hit
CGTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA7550.12341824399541637No Hit
GATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT7510.12276437250404992No Hit
GATATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA7440.12162009739415865No Hit
GGCCTGCGATGTTGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT7440.12162009739415865No Hit
AGTATGAGATGCACCCAGGGTTTTCCCAGTCACGACCCTTCAATCTGAGG7400.1209662259027922No Hit
CCTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACTTAAGAGTCACGCT7390.1208027580299506No Hit
TGCCTGCGATGTTGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT7380.12063929015710896No Hit
ACCCTGCGACAACTCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT7360.12031235441142575No Hit
AGCCTGCGATGTTGCCAGGGTTTTCCCAGTCACGACCCTTCAATCTGAGG7350.12014888653858415No Hit
CACCTGCGAAGGAGCCAGGGTTTTCCCAGTCACGACTTAAGAGTCACGCT7340.11998541866574251No Hit
CTCCTGCGAGGAATCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT7300.11933154717437609No Hit
GTCCTGCGACGAGGCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT7300.11933154717437609No Hit
GACCTGCGACAACTCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT7290.11916807930153446No Hit
GGTATGAGATGCACCCAGGGTTTTCCCAGTCACGACCCTTCAATCTGAGG7270.11884114355585125No Hit
CATATGAGATGCACCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG7240.11835073993732642No Hit
GCTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGTGTCTCCAGAGCA7230.11818727206448482No Hit
TGCCTGCGACGAGGCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT7190.11753340057311837No Hit
CATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT7170.11720646482743513No Hit
CACCTGCGACAACTCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT7160.11704299695459353No Hit
GCTATGAGATAAGCCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG7140.11671606120891032No Hit
GCTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACTTAAGAGTCACGCT7140.11671606120891032No Hit
AGCCTGCGATGTTGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT7050.11524485035333581No Hit
ACTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT7040.11508138248049418No Hit
GTTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGTGTCTCCAGAGCA7040.11508138248049418No Hit
TTTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT7040.11508138248049418No Hit
TGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACTTAAGAGTCACGCT6980.11410057524344454No Hit
CGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT6960.1137736394977613No Hit
TGTATGAGATAAGCCCAGGGTTTTCCCAGTCACGACAGGTCGTTTTTCTT6910.11295630013355325No Hit
CATATGAGATAAGCCCAGGGTTTTCCCAGTCACGACAGGTCGTTTTTCTT6900.11279283226071164No Hit
ATTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT6850.11197549289650359No Hit
CACCTGCGATGTTGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA6820.11148508927797875No Hit
GATATGAGATGCACCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG6790.11099468565945392No Hit
AGTATGAGATGCACCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA6760.11050428204092909No Hit
CTCCTGCGAAGGAGCCAGGGTTTTCCCAGTCACGACTTAAGAGTCACGCT6750.11034081416808747No Hit
CATATGAGATGCACCCAGGGTTTTCCCAGTCACGACGAGGCTGAATTTAT6730.11001387842240426No Hit
CATATGAGATGCACCCAGGGTTTTCCCAGTCACGACAAGACTGAAGGTCA6730.11001387842240426No Hit
CTCCTGCGACGAGGCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT6710.10968694267672102No Hit
AGCCTGCGACAACTCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT6700.10952347480387942No Hit
TGCCTGCGACAACTCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT6690.10936000693103781No Hit
TCTATGAGATAAGCCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG6680.10919653905819621No Hit
CATATGAGATGCACCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA6680.10919653905819621No Hit
GATATGAGATGCACCCAGGGTTTTCCCAGTCACGACAAGACTGAAGGTCA6670.10903307118535457No Hit
GTTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACTTAAGAGTCACGCT6650.10870613543967136No Hit
CGTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGTGTCTCCAGAGCA6630.10837919969398815No Hit
CTTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACTTAAGAGTCACGCT6630.10837919969398815No Hit
CCTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT6590.1077253282026217No Hit
GATATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA6580.10756186032978007No Hit
CACCTGCGACGAGGCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT6570.10739839245693847No Hit
AGTATGAGATGCACCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG6560.10723492458409686No Hit
CGTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA6520.10658105309273042No Hit
AATATGAGATGCACCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG6500.10625411734704719No Hit
ACCCTGCGATGTTGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA6500.10625411734704719No Hit
TGCCTGCGAAGGAGCCAGGGTTTTCCCAGTCACGACTTAAGAGTCACGCT6480.10592718160136398No Hit
CCCCTGCGACAACTCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT6480.10592718160136398No Hit
TTCCTGCGAGGAATCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT6470.10576371372852238No Hit
TTCCTGCGACGAGGCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG6460.10560024585568074No Hit
GCTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT6400.10461943861863109No Hit
GTTATGAGATAAGCCCAGGGTTTTCCCAGTCACGACAGGTCGTTTTTCTT6370.10412903500010624No Hit
AGTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA6350.10380209925442303No Hit
GATATGAGATGCACCCAGGGTTTTCCCAGTCACGACGAGGCTGAATTTAT6340.10363863138158143No Hit
AGTATGAGATAAGCCCAGGGTTTTCCCAGTCACGACAGGTCGTTTTTCTT6310.10314822776305659No Hit
GGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACTTAAGAGTCACGCT6290.10282129201737336No Hit
GACCTGCGAAGGAGCCAGGGTTTTCCCAGTCACGACTTAAGAGTCACGCT6240.10200395265316531No Hit
TACCTGCGACGAGGCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG6190.10118661328895726No Hit
GATATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGTGTCTCCAGAGCA6180.10102314541611565No Hit
TGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT6180.10102314541611565No Hit
AGTATGAGATGCACCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG6170.10085967754327405No Hit
GTCCTGCGAAGGAGCCAGGGTTTTCCCAGTCACGACTTAAGAGTCACGCT6130.1002058060519076No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATGA10600.0245.000021
GATAAGC58550.0245.000028
CATTGAC100.0014760332245.00002245
GCGGGAT100.0014760332245.00002245
TTTATGA10350.0245.000021
GTCCTGC21450.0245.01
TTATGAG49500.0245.02
CGACAAC67300.0245.07
ATGAGAC47350.0245.04
GTATGAG93350.0245.02
CGAAGGA73000.0245.07
GCGACGA69050.0244.999986
ACCTGCG96900.0244.999982
CGTCTTT151.5190231E-5244.99998245
CACCTGC22000.0244.999981
CTGCGAC136400.0244.910194
TATGAGA259300.0244.905523
AGATGCA90400.0244.864497
ATGCACC90350.0244.864419
ATGTTGC80900.0244.848579
Produced by FastQC (version 0.11.5)