FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005466338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005466338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences615585
Sequences flagged as poor quality0
Sequence length251
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAGACCGACGAGGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT16850.2737233688280254No Hit
TAAGACCGACGAGGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT14780.24009681847348457No Hit
TGAGACCGACGAGGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT14020.22775083863317008No Hit
AGAGACCGATGTTGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA12190.19802301875451808No Hit
GGCCCCAGAGGAATCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA11280.18324033236677306No Hit
TTCCCCAGACGAGGCCAGGGTTTTCCCAGTCACGACGACATTCGTTCAAA10320.16764541046321793No Hit
GTAGACCGACGAGGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT10190.1655335981221115No Hit
TGAGACCGATGTTGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA9930.16130997343989864No Hit
GGAGACCGATGTTGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA9830.1596855024082783No Hit
GGAGACCGACGAGGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT9470.15383740669444512No Hit
GAAGACCGAGGAATCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT9310.1512382530438526No Hit
TTAGACCGAGGAATCCAGGGTTTTCCCAGTCACGACGCTGAAAGGACTGG9270.15058846463120446No Hit
TTAGACCGATGTTGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA9150.14863909939326006No Hit
TTAGACCGACGAGGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA9140.14847665229009804No Hit
AGCCCCAGAGGAATCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA9060.14717707546480177No Hit
GAAGACCGAGGAATCCAGGGTTTTCCCAGTCACGACGCTGAAAGGACTGG9060.14717707546480177No Hit
GTAGACCGATGTTGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA8920.14490281602053331No Hit
ATCCCCAGAAGGAGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA8900.14457792181420925No Hit
GAAGACCGACGAGGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT8860.1439281334015611No Hit
TCAGACCGACGAGGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT8780.14262855657626486No Hit
TGCCCCAGAGGAATCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA8680.14100408554464453No Hit
GAAGACCGAGGAATCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC8620.1400294029256723No Hit
GTCCCCAGAGGAATCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA8590.13954206161618624No Hit
TGAGACCGACGAGGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA8460.1374302492750798No Hit
ATAGACCGATGTTGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA8430.13694290796559372No Hit
AACCCCAGAGGAATCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA8390.13629311955294557No Hit
TAAGACCGAGGAATCCAGGGTTTTCCCAGTCACGACGCTGAAAGGACTGG8320.13515598983081134No Hit
GAAGACCGATGTTGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA8300.13483109562448728No Hit
TTCCCCAGAAGGAGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA8280.13450620141816322No Hit
GTCCCCAGAAGGAGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA8280.13450620141816322No Hit
TTAGACCGAGGAATCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT8190.13304417748970493No Hit
TGCCCCAGAAGGAGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA8040.13060747094227443No Hit
TTCCCCAGAGGAATCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA8020.13028257673595034No Hit
ATCCCCAGACGAGGCCAGGGTTTTCCCAGTCACGACGACATTCGTTCAAA7960.12930789411697816No Hit
ATCCCCAGAGGAATCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA7950.12914544701381614No Hit
GTCCCCAGACGAGGCCAGGGTTTTCCCAGTCACGACGACATTCGTTCAAA7870.12784587018851987No Hit
TAAGACCGACGAGGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA7850.1275209759821958No Hit
GTAGACCGAGGAATCCAGGGTTTTCCCAGTCACGACGCTGAAAGGACTGG7770.12622139915689953No Hit
AGCCCCAGAAGGAGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA7690.12492182233160326No Hit
TACCCCAGAGGAATCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA7680.12475937522844124No Hit
TTCCCCAGACGAGGCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCTCAA7640.12410958681579312No Hit
TACCCCAGACGAGGCCAGGGTTTTCCCAGTCACGACGACATTCGTTCAAA7620.12378469260946905No Hit
TACCCCAGAAGGAGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA7550.1226475628873348No Hit
GACCCCAGAGGAATCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA7460.1211855389588765No Hit
AGAGACCGACGAGGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT7430.12069819764939041No Hit
TTAGACCGAAGGAGCCAGGGTTTTCCCAGTCACGACATCGCTGAAGACAG7350.11939862082409416No Hit
AGCCCCAGACGAGGCCAGGGTTTTCCCAGTCACGACGACATTCGTTCAAA7290.11842393820512195No Hit
TTAGACCGAAGGAGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG7230.11744925558614976No Hit
AACCCCAGAAGGAGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA7160.11631212586401553No Hit
AAAGACCGACGAGGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT7130.11582478455452942No Hit
TAAGACCGATGTTGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA7110.11549989034820536No Hit
GACCCCAGATAAGCCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT7090.11517499614188129No Hit
GTAGACCGAAGGAGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG7080.11501254903871927No Hit
ATAGACCGACGAGGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT7070.11485010193555724No Hit
TTAGACCGAGGAATCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC7060.11468765483239521No Hit
ACCCCCAGAGGAATCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA7020.11403786641974707No Hit
GAAGACCGAGGAATCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT6950.11290073669761283No Hit
TGCCCCAGACGAGGCCAGGGTTTTCCCAGTCACGACGACATTCGTTCAAA6950.11290073669761283No Hit
ATAGACCGAAGGAGCCAGGGTTTTCCCAGTCACGACATCGCTGAAGACAG6950.11290073669761283No Hit
GACCCCAGAAGGAGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA6890.11192605407864065No Hit
TAAGACCGAGGAATCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT6880.11176360697547862No Hit
GGCCCCAGAAGGAGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA6800.11046403015018236No Hit
TAAGACCGAAGGAGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG6800.11046403015018236No Hit
TGAGACCGAAGGAGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG6790.11030158304702031No Hit
GAAGACCGAGGAATCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT6750.10965179463437219No Hit
GCCCCCAGAGGAATCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA6690.10867711201539998No Hit
TAAGACCGAGGAATCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC6680.10851466491223795No Hit
TTAGACCGAGGAATCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT6600.10721508808694169No Hit
AAAGACCGATGTTGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA6580.10689019388061761No Hit
CGAGACCGATGTTGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA6570.1067277467774556No Hit
TTAGACCGAGGAATCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA6560.10656529967429357No Hit
AGAGACCGAAGGAGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG6530.10607795836480745No Hit
GTAGACCGAGGAATCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT6520.10591551126164543No Hit
GTAGACCGAAGGAGCCAGGGTTTTCCCAGTCACGACATCGCTGAAGACAG6500.10559061705532136No Hit
ATAGACCGAAGGAGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG6480.10526572284899728No Hit
CGCCCCAGAAGGAGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA6460.10494082864267323No Hit
TAAGACCGAAGGAGCCAGGGTTTTCCCAGTCACGACATCGCTGAAGACAG6440.10461593443634916No Hit
GTCCCCAGATAAGCCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT6430.10445348733318714No Hit
GTAGACCGAGGAATCCAGGGTTTTCCCAGTCACGACGTTTCCCATCAGCC6360.1033163576110529No Hit
TTAGACCGACGAGGCCAGGGTTTTCCCAGTCACGACCAGACCCCAGGACC6350.10315391050789086No Hit
CTCCCCAGAAGGAGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA6330.10282901630156681No Hit
TCCCCCAGAGGAATCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA6320.10266656919840478No Hit
GTCCCCAGACGAGGCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCTCAA6290.10217922788891869No Hit
ATCCCCAGACGAGGCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCTCAA6260.10169188657943257No Hit
GAAGACCGAAGGAGCCAGGGTTTTCCCAGTCACGACATCGCTGAAGACAG6190.10055475685729835No Hit
CGCCCCAGAGGAATCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA6190.10055475685729835No Hit
GTCCCCAGATGTTGCCAGGGTTTTCCCAGTCACGACGGAAGACTAAGTAG6190.10055475685729835No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)