FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005466352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005466352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5388
Sequences flagged as poor quality0
Sequence length251
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACGGCAAGGCCAAAGA3095.734966592427617No Hit
TTCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACACGATACAACATGA3015.58648849294729No Hit
ATCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACACGATACAACATGA2184.046028210838902No Hit
ATCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACGGCAAGGCCAAAGA2093.878990348923534No Hit
GTCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACACGATACAACATGA2023.749072011878248No Hit
GTCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACGGCAAGGCCAAAGA1953.6191536748329622No Hit
CTCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACACGATACAACATGA1863.4521158129175946No Hit
TACGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACGGCAAGGCCAAAGA1653.0623608017817374No Hit
TACGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACACGATACAACATGA1633.0252412769116557No Hit
CTCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACGGCAAGGCCAAAGA1502.783964365256125No Hit
AACGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACGGCAAGGCCAAAGA1412.6169265033407574No Hit
TGCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACACGATACAACATGA1282.3756495916852263No Hit
TCCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACGGCAAGGCCAAAGA1212.2457312546399404No Hit
AACGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACACGATACAACATGA1112.060133630289532No Hit
TGCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACGGCAAGGCCAAAGA1102.0415738678544915No Hit
TCCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACACGATACAACATGA1021.893095768374165No Hit
GACGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACGGCAAGGCCAAAGA961.7817371937639197No Hit
AGCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACACGATACAACATGA931.7260579064587973No Hit
CGCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACGGCAAGGCCAAAGA911.6889383815887158No Hit
CACGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACACGATACAACATGA901.670378619153675No Hit
AGCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACGGCAAGGCCAAAGA881.6332590942835932No Hit
GACGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACACGATACAACATGA861.5961395694135114No Hit
ACCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACGGCAAGGCCAAAGA851.5775798069784706No Hit
ACCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACACGATACAACATGA811.5033407572383073No Hit
CACGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACGGCAAGGCCAAAGA801.4847809948032664No Hit
CGCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACACGATACAACATGA771.429101707498144No Hit
GCCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACACGATACAACATGA741.3734224201930216No Hit
CCCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACGGCAAGGCCAAAGA731.3548626577579805No Hit
GGCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACGGCAAGGCCAAAGA711.317743132887899No Hit
GCCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACGGCAAGGCCAAAGA691.2806236080178173No Hit
GGCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACACGATACAACATGA651.206384558277654No Hit
CCCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACACGATACAACATGA631.1692650334075725No Hit
TTCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG350.6495916852264292No Hit
TTCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACCCGATACAACATGA290.5382331106161842No Hit
ATCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACCCGATACAACATGA240.4454342984409799No Hit
ATCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG220.4083147735708983No Hit
GTCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG200.3711952487008166No Hit
CTCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACCCGATACAACATGA190.3526354862657758No Hit
ATCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACTCGCTTCTCTGCAG190.3526354862657758No Hit
AGCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACCCGATACAACATGA170.31551596139569416No Hit
CTCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACTCGCTTCTCTGCAG170.31551596139569416No Hit
TTCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACTCGCTTCTCTGCAG160.2969561989606533No Hit
GTCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACCCGATACAACATGA160.2969561989606533No Hit
TGCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG160.2969561989606533No Hit
TGCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACCCGATACAACATGA150.27839643652561247No Hit
TACGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACCCGATACAACATGA140.25983667409057165No Hit
CACGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACCCGATACAACATGA140.25983667409057165No Hit
TTCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG130.24127691165553078No Hit
ATCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG130.24127691165553078No Hit
TACGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG130.24127691165553078No Hit
GTCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG130.24127691165553078No Hit
AGCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG120.22271714922048996No Hit
GACGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG110.20415738678544915No Hit
TGCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACTCGCTTCTCTGCAG110.20415738678544915No Hit
CTCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG110.20415738678544915No Hit
ATCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACCCAGTGATCGCTTC100.1855976243504083No Hit
CGCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACCCGATACAACATGA100.1855976243504083No Hit
AACGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACCCGATACAACATGA100.1855976243504083No Hit
AACGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG100.1855976243504083No Hit
TGCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACCCAGTGATCGCTTC100.1855976243504083No Hit
GACGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG100.1855976243504083No Hit
TCCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG100.1855976243504083No Hit
TCCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACCCGATACAACATGA90.16703786191536749No Hit
AGCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG90.16703786191536749No Hit
TGCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG90.16703786191536749No Hit
TACGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACTCGCTTCTCTGCAG90.16703786191536749No Hit
GACGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACCCGATACAACATGA90.16703786191536749No Hit
TACGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACCCAGTGATCGCTTC90.16703786191536749No Hit
GCCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG90.16703786191536749No Hit
TCCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACTCGCTTCTCTGCAG90.16703786191536749No Hit
CTCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACCCAGTGATCGCTTC90.16703786191536749No Hit
ACCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG90.16703786191536749No Hit
GGCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACCCGATACAACATGA80.14847809948032664No Hit
TACGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG80.14847809948032664No Hit
CCCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACCCGATACAACATGA80.14847809948032664No Hit
TTCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACCCAGTGATCGCTTC80.14847809948032664No Hit
GGCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACTCGCTTCTCTGCAG80.14847809948032664No Hit
GTCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACTCGCTTCTCTGCAG80.14847809948032664No Hit
CACGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG80.14847809948032664No Hit
AACGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACCCAGTGATCGCTTC80.14847809948032664No Hit
TTCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACCTCCCTGTTTATCC80.14847809948032664No Hit
CCCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACTCGCTTCTCTGCAG70.12991833704528583No Hit
ACCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACCCGATACAACATGA70.12991833704528583No Hit
CTCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG70.12991833704528583No Hit
AGCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACTCGCTTCTCTGCAG70.12991833704528583No Hit
TTCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA70.12991833704528583No Hit
AACGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACTCGCTTCTCTGCAG70.12991833704528583No Hit
CCCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG60.11135857461024498No Hit
GGCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG60.11135857461024498No Hit
CGCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG60.11135857461024498No Hit
GGCGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG60.11135857461024498No Hit
CACGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACTCGCTTCTCTGCAG60.11135857461024498No Hit
CACGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG60.11135857461024498No Hit
AACGGTTGACTCAGCCAGGGTTTTCCCAGTCACGACGCGATACAACATGA60.11135857461024498No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGACT5350.0245.000025
GGTTGAC5350.0245.000024
CCAGTGA100.0014355029245.00002245
TGACTCA5350.0245.000027
GCCGGTT100.0014355029245.000021
TTGACTC5350.0245.000026
CGCGGTT201.4220313E-7245.000021
CGGTTGA5350.0245.000023
ACCGGTT251.4024408E-9245.01
CCCGGTT151.4365207E-5245.01
TCGGTTG2400.0245.02
TACGGTT350.0245.01
GCGGTTG1000.0245.02
GACGGTT251.4024408E-9245.01
TCCGGTT151.4365207E-5245.01
ATCGGTT350.0245.01
CTCGGTT750.0245.01
ACGGTTG1300.0245.02
TTCGGTT650.0245.01
TGCGGTT350.0245.01
Produced by FastQC (version 0.11.5)