FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005466353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005466353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5388
Sequences flagged as poor quality0
Sequence length251
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTCAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA97018.002969561989605No Hit
TGGTTCAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA71913.344469190794358No Hit
AGGTTCAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA67512.52783964365256No Hit
GTGTTCAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA4257.887899034892354No Hit
TTGTTCAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA3536.551596139569414No Hit
GAGTTCAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA3085.716406829992576No Hit
TAGTTCAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA2594.806978470675576No Hit
GCGTTCAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1993.6933927245731253No Hit
CGGTTCAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1873.4706755753526357No Hit
ATGTTCAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1572.913882702301411No Hit
TCGTTCAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1372.542687453600594No Hit
TGGTTCAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG771.429101707498144No Hit
TCGTTCAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG751.3919821826280625No Hit
CAGTTCAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA721.3363028953229399No Hit
ACGTTCAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA601.1135857461024499No Hit
CTGTTCAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA581.0764662212323683No Hit
AGGTTCAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG480.8908685968819599No Hit
CAGTTCAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG470.8723088344469191No Hit
TAGTTCAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG470.8723088344469191No Hit
CGGTTCAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG460.8537490720118782No Hit
AAGTTCAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA460.8537490720118782No Hit
GTGTTCAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG420.779510022271715No Hit
ACGTTCAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG420.779510022271715No Hit
GGGTTCAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG350.6495916852264292No Hit
GCGTTCAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG270.5011135857461024No Hit
AAGTTCAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG260.48255382331106156No Hit
TTGTTCAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG250.46399406087602074No Hit
CCGTTCAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG230.4268745360059391No Hit
CCGTTCAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA230.4268745360059391No Hit
ATGTTCAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG140.25983667409057165No Hit
GAGTTCAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG140.25983667409057165No Hit
CTGTTCAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG100.1855976243504083No Hit
GGGTTCAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGATA60.11135857461024498No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTCAG2850.0245.000022
TCGTTCA100.0014355029245.000021
CGTTCAG600.0245.02
TTGTTCA350.0245.01
TGGTTCA750.0245.01
GTCACTG4400.0245.08
GTGTTCA251.4024408E-9245.01
GGGTTCA1050.0245.01
CAGTCAC4400.0245.06
ACGTTCA151.4365207E-5245.01
TGTTCAG950.0245.02
GTTCAGT4400.0245.03
GTTCAGA950.0245.03
TAGTTCA450.0245.01
GAGTTCA450.0245.01
TCAGTCA4400.0245.05
TCAGAGA950.0245.05
TGTAGGG700.0245.0245
GCGTTCA301.4551915E-11245.01
ATGTTCA301.4551915E-11245.01
Produced by FastQC (version 0.11.5)