FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005466358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005466358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences402723
Sequences flagged as poor quality0
Sequence length251
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGGTGACTCAGCCAGGGTTTTCCCAGTCACGACGGAAGACTAAGTAG17170.4263476384512432No Hit
GGAAGGTGATGTTGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT17020.422622993968559No Hit
GGAAGGTGATGTTGCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCTCAA12740.31634647139597194No Hit
GGAAGGTGACTCAGCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCTCAA12330.3061657764766353No Hit
GAAAGGTGACTCAGCCAGGGTTTTCCCAGTCACGACGGAAGACTAAGTAG10300.2557589211443101No Hit
GTAAGGTGACTCAGCCAGGGTTTTCCCAGTCACGACGGAAGACTAAGTAG9270.2301830290298791No Hit
GAAAGGTGATGTTGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT9260.2299347193977002No Hit
GCAAGGTGATGTTGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT9210.22869317123680546No Hit
GTAAGGTGATGTTGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT9120.22645838454719494No Hit
CGAAGGTGACTCAGCCAGGGTTTTCCCAGTCACGACGGAAGACTAAGTAG8990.22323035932886875No Hit
TTAAGGTGACAACTCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT8910.2212438822714372No Hit
GTAAGGTGACAACTCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT8710.21627768962785834No Hit
GGAAGGTGATGTTGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA8680.21553276073132152No Hit
TAAAGGTGACAACTCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT8640.21453952220260578No Hit
TGAAGGTGACAACTCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT8440.2095733295590269No Hit
AGAAGGTGACTCAGCCAGGGTTTTCCCAGTCACGACGGAAGACTAAGTAG8300.20609699470852172No Hit
TCAAGGTGACAACTCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT8160.20262065985801656No Hit
GGAAGGTGATGTTGCCAGGGTTTTCCCAGTCACGACAAGACTGAAGGTCA8150.20237235022583763No Hit
TGAAGGTGACTCAGCCAGGGTTTTCCCAGTCACGACGGAAGACTAAGTAG7950.19740615758225877No Hit
AAAAGGTGACAACTCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT7630.18946024935253264No Hit
TCAAGGTGAGGAATCCAGGGTTTTCCCAGTCACGACGACATTCGTTCAAA7440.18474236634113272No Hit
GAAAGGTGACTCAGCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCTCAA7430.18449405670895377No Hit
TCAAGGTGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT7350.18250757965152228No Hit
GGAAGGTGACTCAGCCAGGGTTTTCCCAGTCACGACAAGACTGAAGGTCA7300.18126603149062756No Hit
GTAAGGTGACTCAGCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCTCAA7270.18052110259409074No Hit
CAAAGGTGACAACTCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT7070.1755549099505119No Hit
GCAAGGTGATGTTGCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCTCAA7070.1755549099505119No Hit
AGAAGGTGATGTTGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT6980.1733201232609014No Hit
CGAAGGTGACTCAGCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCTCAA6970.17307181362872248No Hit
TCAAGGTGAGGAATCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA6910.1715819558356488No Hit
GAAAGGTGATGTTGCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCTCAA6880.17083702693911199No Hit
TTAAGGTGATGCACCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT6780.1683539306173226No Hit
GGAAGGTGACTCAGCCAGGGTTTTCCCAGTCACGACCCCTGATGGCTACA6780.1683539306173226No Hit
GTAAGGTGATGTTGCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCTCAA6670.16562252466335423No Hit
TGAAGGTGATGTTGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT6460.16040802238759644No Hit
ATAAGGTGACAACTCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT6430.15966309349105962No Hit
TGAAGGTGACTCAGCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCTCAA6390.15866985496234384No Hit
GAAAGGTGACAACTCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT6250.15519352011183868No Hit
ACAAGGTGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT6230.15469690084748078No Hit
AAAAGGTGATAAGCCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG6180.15345535268658608No Hit
TCAAGGTGATGTTGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT6140.1524621141578703No Hit
AGAAGGTGACTCAGCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCTCAA6010.14923408893954404No Hit
AGAAGGTGACAACTCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT5890.14625437335339675No Hit
GCAAGGTGACTCAGCCAGGGTTTTCCCAGTCACGACGGAAGACTAAGTAG5880.1460060637212178No Hit
CTAAGGTGACAACTCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT5880.1460060637212178No Hit
GGAAGGTGATAAGCCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG5850.14526113482468098No Hit
CCAAGGTGAGGAATCCAGGGTTTTCCCAGTCACGACGACATTCGTTCAAA5750.14277803850289156No Hit
ACAAGGTGAGGAATCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA5720.14203310960635473No Hit
ACAAGGTGATGTTGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT5710.1417847999741758No Hit
GGAAGGTGACTCAGCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT5680.141039871077639No Hit
CCAAGGTGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT5670.14079156144546004No Hit
ACAAGGTGACGAGGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA5620.13955001328456532No Hit
TCAAGGTGACGAGGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA5570.13830846512367062No Hit
AGAAGGTGATGTTGCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCTCAA5560.13806015549149167No Hit
CAAAGGTGACTCAGCCAGGGTTTTCCCAGTCACGACGGAAGACTAAGTAG5480.13607367843406012No Hit
ACAAGGTGAGGAATCCAGGGTTTTCCCAGTCACGACGACATTCGTTCAAA5470.13582536880188117No Hit
TTAAGGTGATGCACCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT5300.1316041050548392No Hit
CCAAGGTGACGAGGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA5270.13085917615830236No Hit
GCAAGGTGACAACTCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT5270.13085917615830236No Hit
TGAAGGTGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT5210.1293693183652287No Hit
AAAAGGTGACTCAGCCAGGGTTTTCCCAGTCACGACGGAAGACTAAGTAG5200.12912100873304974No Hit
GCAAGGTGACGAGGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA5070.12589298351472353No Hit
CCAAGGTGAGGAATCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA5050.12539636425036563No Hit
GAAAGGTGATAAGCCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG5040.1251480546181867No Hit
TCAAGGTGATGTTGCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCTCAA5030.12489974498600775No Hit
TAAAGGTGATGCACCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT5030.12489974498600775No Hit
GTAAGGTGATAAGCCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG5020.1246514353538288No Hit
TTAAGGTGACAACTCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA4990.12390650645729198No Hit
TTAAGGTGATGCACCCAGGGTTTTCCCAGTCACGACTCTCACCTAAATCT4960.12316157756075516No Hit
TGAAGGTGATGTTGCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCTCAA4930.12241664866421834No Hit
GTAAGGTGACTCAGCCAGGGTTTTCCCAGTCACGACAAGACTGAAGGTCA4910.12192002939986045No Hit
CCAAGGTGACAACTCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT4910.12192002939986045No Hit
TGAAGGTGACGAGGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA4910.12192002939986045No Hit
CGAAGGTGATGTTGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4860.12067848123896573No Hit
GCAAGGTGACTCAGCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCTCAA4780.11869200418153422No Hit
GCAAGGTGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4760.11819538491717632No Hit
AAAAGGTGACAACTCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT4760.11819538491717632No Hit
GCAAGGTGATGTTGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA4740.11769876565281845No Hit
TAAAGGTGACAACTCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG4720.11720214638846055No Hit
TTAAGGTGACAACTCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT4710.1169538367562816No Hit
GTAAGGTGACAACTCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT4690.11645721749192373No Hit
TTAAGGTGACAACTCCAGGGTTTTCCCAGTCACGACGTCTCCAGATCAAC4680.11620890785974478No Hit
GCAAGGTGATGTTGCCAGGGTTTTCCCAGTCACGACAAGACTGAAGGTCA4660.1157122885953869No Hit
TTAAGGTGATGTTGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4640.11521566933102902No Hit
GTAAGGTGATGCACCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4600.11422243080231324No Hit
ACAAGGTGACAACTCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT4560.11322919227359747No Hit
TAAAGGTGATGTTGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4480.11124271521616597No Hit
CGAAGGTGACAACTCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT4480.11124271521616597No Hit
CTAAGGTGACTCAGCCAGGGTTTTCCCAGTCACGACGGAAGACTAAGTAG4470.11099440558398702No Hit
GTAAGGTGACAACTCCAGGGTTTTCCCAGTCACGACGTCTCCAGATCAAC4460.11074609595180807No Hit
GTAAGGTGATGTTGCCAGGGTTTTCCCAGTCACGACAAGACTGAAGGTCA4450.11049778631962912No Hit
TAAAGGTGACAACTCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA4430.11000116705527124No Hit
GGAAGGTGATGTTGCCAGGGTTTTCCCAGTCACGACAAACAAGACCAAAG4400.10925623815873442No Hit
TAAAGGTGACAACTCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT4390.10900792852655547No Hit
TTAAGGTGACAACTCCAGGGTTTTCCCAGTCACGACTACAAAGTCTCTCG4360.10826299963001866No Hit
TAAAGGTGACAACTCCAGGGTTTTCCCAGTCACGACGTCTCCAGATCAAC4350.10801468999783971No Hit
TCAAGGTGACAACTCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT4350.10801468999783971No Hit
GCAAGGTGATAAGCCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG4330.10751807073348181No Hit
TTAAGGTGAGGAATCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA4320.10726976110130289No Hit
ACAAGGTGAAGGAGCCAGGGTTTTCCCAGTCACGACGTTTGGAGAAGCAA4300.10677314183694499No Hit
TAAAGGTGACTCAGCCAGGGTTTTCCCAGTCACGACGGAAGACTAAGTAG4300.10677314183694499No Hit
CGAAGGTGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4290.10652483220476607No Hit
TGAAGGTGACTCAGCCAGGGTTTTCCCAGTCACGACAAGACTGAAGGTCA4290.10652483220476607No Hit
AAAAGGTGATGTTGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4290.10652483220476607No Hit
AAAAGGTGATAAGCCCAGGGTTTTCCCAGTCACGACGGCAAGGCCAAAGA4250.10553159367605029No Hit
TGAAGGTGACAACTCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT4230.1050349744116924No Hit
TAAAGGTGATAAGCCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG4230.1050349744116924No Hit
ACAAGGTGAAGGAGCCAGGGTTTTCCCAGTCACGACACGACTGTCGCTAC4220.10478666477951346No Hit
AAAAGGTGACAACTCCAGGGTTTTCCCAGTCACGACGTCTCCAGATCAAC4210.10453835514733453No Hit
ATAAGGTGACTCAGCCAGGGTTTTCCCAGTCACGACGGAAGACTAAGTAG4200.10429004551515558No Hit
GCAAGGTGAGGAATCCAGGGTTTTCCCAGTCACGACGACATTCGTTCAAA4200.10429004551515558No Hit
TTAAGGTGATGCACCCAGGGTTTTCCCAGTCACGACCTCAGCAGAGATGC4190.10404173588297663No Hit
TTAAGGTGACTCAGCCAGGGTTTTCCCAGTCACGACGGAAGACTAAGTAG4180.10379342625079768No Hit
TGAAGGTGACAACTCCAGGGTTTTCCCAGTCACGACGTCTCCAGATCAAC4180.10379342625079768No Hit
GCAAGGTGAGGAATCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA4170.10354511661861876No Hit
TCAAGGTGACAACTCCAGGGTTTTCCCAGTCACGACGTCTCCAGATCAAC4160.10329680698643981No Hit
TAAAGGTGAGGAATCCAGGGTTTTCCCAGTCACGACGACATTCGTTCAAA4150.10304849735426086No Hit
TTAAGGTGAGGAATCCAGGGTTTTCCCAGTCACGACGACATTCGTTCAAA4150.10304849735426086No Hit
TGAAGGTGACAACTCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA4140.10280018772208194No Hit
GTAAGGTGACAACTCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA4140.10280018772208194No Hit
GTAAGGTGACTCAGCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT4130.10255187808990299No Hit
AAAAGGTGACTCAGCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCTCAA4110.1020552588255451No Hit
ACAAGGTGATGTTGCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCTCAA4110.1020552588255451No Hit
GAAAGGTGACTCAGCCAGGGTTTTCCCAGTCACGACAAGACTGAAGGTCA4110.1020552588255451No Hit
GAAAGGTGATGTTGCCAGGGTTTTCCCAGTCACGACAAGACTGAAGGTCA4110.1020552588255451No Hit
ATAAGGTGAAGGAGCCAGGGTTTTCCCAGTCACGACGTTTGGAGAAGCAA4080.10131032992900828No Hit
GGAAGGTGATAAGCCCAGGGTTTTCCCAGTCACGACGGCAAGGCCAAAGA4050.10056540103247145No Hit
ATAAGGTGATGTTGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4050.10056540103247145No Hit
GGAAGGTGACTCAGCCAGGGTTTTCCCAGTCACGACGGCAAGGCCAAAGA4050.10056540103247145No Hit
CGAAGGTGACTCAGCCAGGGTTTTCCCAGTCACGACAAGACTGAAGGTCA4030.10006878176811355No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTTGC58150.0244.999159
ACAACTC75450.0244.999139
TTAAGGT27450.0244.999131
TGACAAC75450.0244.999137
GATGCAC35000.0244.999138
GGTGACG43350.0244.999135
GTAAGGT30400.0244.999131
GTGACGA43350.0244.999136
ATGCACC35000.0244.999139
AGGTGAC180700.0244.931354
TAAGGTG101650.0244.878622
AAGGTGA402950.0244.847143
GTGACAA75500.0244.836886
GATGTTG58200.0244.788658
TGATGTT58200.0244.788657
TGATAAG53250.0244.76917
GTGATAA53250.0244.76916
ATAAGCC53250.0244.76919
TGACGAG43400.0244.716877
AGGTGAA41400.0244.703234
Produced by FastQC (version 0.11.5)