FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005466372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005466372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519625
Sequences flagged as poor quality0
Sequence length251
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGTAAGACAACTCCAGGGTTTTCCCAGTCACGACCCTTCAATCTGAGG14490.27885494346884776No Hit
TACGTAAGACAACTCCAGGGTTTTCCCAGTCACGACCCTTCAATCTGAGG14040.2701948520567717No Hit
TTCGTAAGAAGGAGCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT13210.25422179456338706No Hit
ATCGTAAGACAACTCCAGGGTTTTCCCAGTCACGACCCTTCAATCTGAGG13190.2538369016117393No Hit
AACGTAAGACAACTCCAGGGTTTTCCCAGTCACGACCCTTCAATCTGAGG12930.24883329324031753No Hit
GGCGTAAGATGTTGCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA12520.24094298773153716No Hit
ATCGTAAGAAGGAGCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT11720.22554726966562424No Hit
ATCGTAAGATGCACCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA11700.22516237671397643No Hit
CTCGTAAGAAGGAGCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT11430.21996632186673085No Hit
AACGTAAGATGCACCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA11270.21688717825354822No Hit
TCCGTAAGAAGGAGCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT11170.2149627134953091No Hit
GGCGTAAGACGAGGCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG10870.20918931922059178No Hit
AGCGTAAGATGTTGCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA10860.20899687274476786No Hit
ACCGTAAGAAGGAGCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT10720.2063026220832331No Hit
AGCGTAAGACAACTCCAGGGTTTTCCCAGTCACGACCCTTCAATCTGAGG10670.20534038970411356No Hit
GACGTAAGATGTTGCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA10610.20418571084917006No Hit
TGCGTAAGATGTTGCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA10550.20303103199422662No Hit
TACGTAAGAAGGAGCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT10520.20245369256675486No Hit
GTCGTAAGAAGGAGCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT10490.20187635313928315No Hit
GGCGTAAGACTCAGCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA10450.2011065672359875No Hit
TTCGTAAGATGCACCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA10290.1980274236228049No Hit
AGCGTAAGACTCAGCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA10260.1974500841953332No Hit
TGCGTAAGACAACTCCAGGGTTTTCCCAGTCACGACCCTTCAATCTGAGG10190.19610295886456577No Hit
AACGTAAGATGTTGCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA10170.195718065912918No Hit
GTCGTAAGATAAGCCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG10130.19494828000962233No Hit
AACGTAAGAAGGAGCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT10070.19379360115467886No Hit
GGCGTAAGATAAGCCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG9960.19167668992061582No Hit
AGCGTAAGACGAGGCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG9640.18551840269425066No Hit
GACGTAAGATAAGCCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG9600.18474861679095503No Hit
TACGTAAGATGCACCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA9520.18320904498436372No Hit
CCCGTAAGAAGGAGCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT9480.18243925908106806No Hit
AGCGTAAGACTCAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT9460.18205436612942025No Hit
CACGTAAGAAGGAGCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT9270.17839788308876592No Hit
TCCGTAAGACAACTCCAGGGTTTTCCCAGTCACGACCCTTCAATCTGAGG8950.17223959586240076No Hit
GTCGTAAGACGAGGCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG8820.16973779167668993No Hit
GGCGTAAGATGTTGCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT8790.1691604522492182No Hit
TACGTAAGATGTTGCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA8750.16839066634592254No Hit
TTCGTAAGACAACTCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA8750.16839066634592254No Hit
TGCGTAAGACTCAGCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA8690.16723598749097907No Hit
CGCGTAAGATGTTGCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA8470.16300216502285303No Hit
AGCGTAAGATGTTGCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT8450.16261727207120522No Hit
AGCGTAAGATAAGCCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG8390.16146259321626172No Hit
GTCGTAAGACTCAGCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA8380.16127014674043783No Hit
ATCGTAAGATAAGCCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG8380.16127014674043783No Hit
GGCGTAAGACTCAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT8380.16127014674043783No Hit
GCCGTAAGATGTTGCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA8290.1595381284580226No Hit
CTCGTAAGATGCACCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA8200.1578061101756074No Hit
GCCGTAAGAAGGAGCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT8130.15645898484484003No Hit
TACGTAAGACAACTCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA8030.15453452008660093No Hit
GACGTAAGAAGGAGCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT7910.152225162376714No Hit
ATCGTAAGACAACTCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA7870.15145537647341833No Hit
GTCGTAAGATGTTGCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA7850.1510704835217705No Hit
ACCGTAAGATGCACCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA7790.14991580466682702No Hit
TGCGTAAGATGTTGCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT7730.14876112581188358No Hit
AACGTAAGACAACTCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA7730.14876112581188358No Hit
AACGTAAGACTCAGCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA7700.14818378638441185No Hit
CGCGTAAGACTCAGCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA7700.14818378638441185No Hit
TGCGTAAGAAGGAGCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT7700.14818378638441185No Hit
ACCGTAAGACAACTCCAGGGTTTTCCCAGTCACGACCCTTCAATCTGAGG7690.14799133990858793No Hit
GCCGTAAGATAAGCCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG7620.14664421457782054No Hit
GTCGTAAGATAAGCCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA7610.14645176810199664No Hit
AGCGTAAGAAGGAGCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT7590.14606687515034883No Hit
TGCGTAAGATGCACCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA7570.145681982198701No Hit
ATCGTAAGACTCAGCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA7560.14548953572287707No Hit
AGCGTAAGATGCACCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA7530.14491219629540533No Hit
CTCGTAAGACAACTCCAGGGTTTTCCCAGTCACGACCCTTCAATCTGAGG7390.14221794563387058No Hit
ATCGTAAGATGTTGCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA7370.14183305268222277No Hit
TCCGTAAGATGCACCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA7360.14164060620639884No Hit
CACGTAAGATGCACCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA7320.1408708203031032No Hit
TTCGTAAGACTCAGCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA7270.13990858792398364No Hit
GACGTAAGATGTTGCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT7230.13913880202068798No Hit
GGCGTAAGAAGGAGCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT7210.13875390906904017No Hit
CACGTAAGATGTTGCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA7200.13856146259321625No Hit
CGCGTAAGAAGGAGCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT7180.13817656964156844No Hit
CACGTAAGACAACTCCAGGGTTTTCCCAGTCACGACCCTTCAATCTGAGG7150.1375992302140967No Hit
GTCGTAAGACAACTCCAGGGTTTTCCCAGTCACGACCCTTCAATCTGAGG7140.13740678373827278No Hit
TTCGTAAGACAACTCCAGGGTTTTCCCAGTCACGACGGAACGATTTTCTG7070.13605965840750542No Hit
GACGTAAGATGCACCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA7070.13605965840750542No Hit
TGCGTAAGACGAGGCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG7060.1358672119316815No Hit
TGCGTAAGACTCAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT7050.1356747654558576No Hit
GTCGTAAGATGCACCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA7030.13528987250420976No Hit
GACGTAAGATAAGCCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA6970.1341351936492663No Hit
CGCGTAAGACAACTCCAGGGTTTTCCCAGTCACGACCCTTCAATCTGAGG6910.13298051479432282No Hit
GACGTAAGACAACTCCAGGGTTTTCCCAGTCACGACCCTTCAATCTGAGG6780.130478710608612No Hit
TTCGTAAGATAAGCCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG6730.12951647822949242No Hit
GTCGTAAGACTCAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT6720.1293240317536685No Hit
GACGTAAGACGAGGCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG6680.12855424585037287No Hit
GGCGTAAGATAAGCCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA6630.12759201347125332No Hit
CGCGTAAGACTCAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT6630.12759201347125332No Hit
GGCGTAAGATGTTGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG6570.12643733461630985No Hit
AACGTAAGATGTTGCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT6510.12528265576136635No Hit
ATCGTAAGACAACTCCAGGGTTTTCCCAGTCACGACGGAACGATTTTCTG6510.12528265576136635No Hit
AACGTAAGACAACTCCAGGGTTTTCCCAGTCACGACGGAACGATTTTCTG6430.12374308395477508No Hit
TACGTAAGACAACTCCAGGGTTTTCCCAGTCACGACGGAACGATTTTCTG6430.12374308395477508No Hit
ATCGTAAGACTCAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT6420.12355063747895116No Hit
ACCGTAAGATGTTGCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA6410.12335819100312727No Hit
AACGTAAGACGAGGCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG6380.12278085157565552No Hit
TTCGTAAGATGTTGCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA6350.12220351214818378No Hit
AGCGTAAGACAACTCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA6280.12085638681741641No Hit
ATCGTAAGACGAGGCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG6260.12047149386576858No Hit
GGCGTAAGACGAGGCCAGGGTTTTCCCAGTCACGACCCCTGATGGCTACA6220.11970170796247294No Hit
TGCGTAAGACAACTCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA6220.11970170796247294No Hit
AACGTAAGACTCAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT6080.11700745730093819No Hit
AACGTAAGATAAGCCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG6060.11662256434929036No Hit
GCCGTAAGATGCACCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA6050.11643011787346644No Hit
TGCGTAAGATAAGCCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG5990.11527543901852298No Hit
AGCGTAAGACTCAGCCAGGGTTTTCCCAGTCACGACGCTAAGATGCCTAA5910.11373586721193168No Hit
CGCGTAAGATGTTGCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT5880.11315852778445994No Hit
TACGTAAGACTCAGCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA5860.11277363483281212No Hit
AGCGTAAGATAAGCCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA5850.11258118835698822No Hit
TTCGTAAGACTCAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT5820.11200384892951648No Hit
GCCGTAAGATGTTGCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT5770.11104161655039692No Hit
CTCGTAAGATAAGCCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG5740.11046427712292517No Hit
AGCGTAAGATGTTGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG5690.10950204474380563No Hit
ATCGTAAGATAAGCCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA5680.10930959826798171No Hit
GGCGTAAGATGCACCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA5660.1089247053163339No Hit
AGCGTAAGACGAGGCCAGGGTTTTCCCAGTCACGACCCCTGATGGCTACA5630.10834736588886215No Hit
TCCGTAAGATGTTGCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA5620.10815491941303826No Hit
GCCGTAAGATAAGCCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA5600.10777002646139042No Hit
ACCGTAAGATAAGCCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG5590.10757757998556651No Hit
GACGTAAGACTCAGCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA5580.10738513350974259No Hit
TACGTAAGATGTTGCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT5570.1071926870339187No Hit
TTCGTAAGACGAGGCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG5560.10700024055809478No Hit
TTCGTAAGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT5430.10449843637238393No Hit
CTCGTAAGACTCAGCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA5400.1039210969449122No Hit
GTCGTAAGATGTTGCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT5380.10353620399326438No Hit
CACGTAAGACGAGGCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG5310.10218907866249699No Hit
TCCGTAAGACAACTCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA5290.10180418571084918No Hit
GTCGTAAGACGAGGCCAGGGTTTTCCCAGTCACGACCCCTGATGGCTACA5290.10180418571084918No Hit
GCCGTAAGACGAGGCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG5290.10180418571084918No Hit
ATCGTAAGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT5280.10161173923502526No Hit
CCCGTAAGATGTTGCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA5270.10141929275920135No Hit
CGCGTAAGATAAGCCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG5260.10122684628337743No Hit
CACGTAAGACTCAGCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA5230.1006495068559057No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAA39550.0245.000021
CCCGTAA17900.0245.000021
ATGCGAG100.0014759694245.00002245
CACGTAA29550.0245.000021
TGCGTAA37200.0245.000021
AACGTAA37600.0245.01
GACGTAA30100.0245.01
GGCGTAA37400.0245.01
AGACGAG77150.0245.07
GTCGTAA37550.0245.01
ACGTAAG130700.0245.02
CGCGTAA28300.0245.01
GCCGTAA26400.0245.01
TACGTAA33450.0245.01
GATGCAC38600.0244.999988
CGTAAGA519650.0244.976413
TCGTAAG148050.0244.917252
GTAAGAC238350.0244.89724
AAGACGA77200.0244.841316
GCGTAAG143150.0244.828862
Produced by FastQC (version 0.11.5)