FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005466378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005466378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences272577
Sequences flagged as poor quality0
Sequence length251
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACTACGATAAGCCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT14670.5381965462970096No Hit
TGACTACGATAAGCCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT11630.4266684276369613No Hit
TGACTACGAGGAATCCAGGGTTTTCCCAGTCACGACCTGTGAACTTCCAG9300.34118799458501636No Hit
GGACTACGATAAGCCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT8910.32688011094112857No Hit
AGACTACGATAAGCCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT8750.32101020995902074No Hit
TGACTACGAGGAATCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA8630.3166077842224399No Hit
TGACTACGATAAGCCCAGGGTTTTCCCAGTCACGACTGGCTACAATGTCT8570.31440657135414946No Hit
TGACTACGAGGAATCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT8110.2975306060305895No Hit
TGACTACGATAAGCCCAGGGTTTTCCCAGTCACGACTCCAGAAGGCAAGA7980.2927613114826269No Hit
GGACTACGAGGAATCCAGGGTTTTCCCAGTCACGACCTGTGAACTTCCAG7520.27588534615906696No Hit
TGACTACGATAAGCCCAGGGTTTTCCCAGTCACGACGGCTACAATGTATC7430.27258352685663134No Hit
GGACTACGATAAGCCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT7090.2601099872696523No Hit
TGACTACGATAAGCCCAGGGTTTTCCCAGTCACGACCCCTGATGGCTACA6980.25607443034445315No Hit
GGACTACGAGGAATCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA6860.25167200460787226No Hit
TGACTACGATAAGCCCAGGGTTTTCCCAGTCACGACCTCTGAGCTGAATG6760.2480033164940549No Hit
CGACTACGAGGAATCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA6690.2454352348143827No Hit
GGACTACGAGGAATCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT6670.24470149719161924No Hit
CGACTACGAGGAATCCAGGGTTTTCCCAGTCACGACCTGTGAACTTCCAG6650.24396775956885577No Hit
TGACTACGATAAGCCCAGGGTTTTCCCAGTCACGACAAGACTGAAGGTCA6540.23993220264365667No Hit
AGACTACGATAAGCCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT6400.2347960392843123No Hit
CGACTACGAGGAATCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT6280.23039361354773147No Hit
TGACTACGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA5850.21461825465831672No Hit
TGACTACGATAAGCCCAGGGTTTTCCCAGTCACGACGCTCTGAGATGAAT5500.2017778462599559No Hit
AGACTACGAGGAATCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA5420.19884289576890202No Hit
GGACTACGATAAGCCCAGGGTTTTCCCAGTCACGACTGGCTACAATGTCT5310.19480733884370288No Hit
AGACTACGAGGAATCCAGGGTTTTCCCAGTCACGACCTGTGAACTTCCAG5180.19003804429574028No Hit
TGACTACGATAAGCCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG5090.18673622499330464No Hit
TGACTACGATAAGCCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG5060.18563561855915944No Hit
AGACTACGATAAGCCCAGGGTTTTCCCAGTCACGACTGGCTACAATGTCT4950.1816000616339603No Hit
GAACTACGAGGAATCCAGGGTTTTCCCAGTCACGACCTGTGAACTTCCAG4950.1816000616339603No Hit
AGACTACGAGGAATCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT4910.18013258638843335No Hit
CGACTACGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA4730.17352894778356207No Hit
GGACTACGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA4730.17352894778356207No Hit
TGACTACGAGGAATCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG4730.17352894778356207No Hit
GGACTACGATAAGCCCAGGGTTTTCCCAGTCACGACCCCTGATGGCTACA4690.1720614725380351No Hit
GGACTACGATAAGCCCAGGGTTTTCCCAGTCACGACGGCTACAATGTATC4580.16802591561283603No Hit
GGACTACGATAAGCCCAGGGTTTTCCCAGTCACGACTCCAGAAGGCAAGA4580.16802591561283603No Hit
GAACTACGAGGAATCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT4510.16545783393316385No Hit
GAACTACGAGGAATCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA4410.16178914581934645No Hit
GTACTACGAGGAATCCAGGGTTTTCCCAGTCACGACCTGTGAACTTCCAG4410.16178914581934645No Hit
GGACTACGATAAGCCCAGGGTTTTCCCAGTCACGACCTCTGAGCTGAATG4380.16068853938520122No Hit
TGACTACGATAAGCCCAGGGTTTTCCCAGTCACGACGCCTGATGGATCAA4370.16032167057381952No Hit
TAACTACGAGGAATCCAGGGTTTTCCCAGTCACGACCTGTGAACTTCCAG4360.1599548017624378No Hit
TGACTACGAGGAATCCAGGGTTTTCCCAGTCACGACCTCCGCACAACAGT4310.15812045770552907No Hit
GTACTACGATGCACCCAGGGTTTTCCCAGTCACGACTCTCACCTAAATCT4130.15151681910065778No Hit
TTACTACGACTCAGCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT4120.15114995028927605No Hit
AGACTACGATAAGCCCAGGGTTTTCCCAGTCACGACGGCTACAATGTATC4060.14894873742098563No Hit
GGACTACGATAAGCCCAGGGTTTTCCCAGTCACGACAAGACTGAAGGTCA4050.1485818686096039No Hit
TAACTACGACTCAGCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT4040.14821499979822214No Hit
AGACTACGATAAGCCCAGGGTTTTCCCAGTCACGACTCCAGAAGGCAAGA4010.14711439336407695No Hit
TAACTACGACTCAGCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG3960.14528004930716823No Hit
GTACTACGACTCAGCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT3900.1430788364388778No Hit
GTACTACGACTCAGCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG3860.14161136119335088No Hit
GTACTACGACTCAGCCAGGGTTTTCCCAGTCACGACCCCTGATGGCTACA3850.14124449238196912No Hit
TTACTACGACTCAGCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG3840.1408776235705874No Hit
TGACTACGATAAGCCCAGGGTTTTCCCAGTCACGACACTAACCTTTCAGT3840.1408776235705874No Hit
GTACTACGAGGAATCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA3810.13977701713644217No Hit
AGACTACGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA3790.1390432795136787No Hit
GAACTACGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA3780.13867641070229697No Hit
AGACTACGATAAGCCCAGGGTTTTCCCAGTCACGACCCCTGATGGCTACA3770.13830954189091524No Hit
TAACTACGAGGAATCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT3760.13794267307953348No Hit
GGACTACGAGGAATCCAGGGTTTTCCCAGTCACGACCTCCGCACAACAGT3730.13684206664538828No Hit
GCACTACGAGGAATCCAGGGTTTTCCCAGTCACGACCTGTGAACTTCCAG3700.13574146021124306No Hit
TAACTACGAGGAATCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA3670.13464085377709784No Hit
TTACTACGAGGAATCCAGGGTTTTCCCAGTCACGACCTGTGAACTTCCAG3660.1342739849657161No Hit
GTACTACGAGGAATCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT3590.13170590328604395No Hit
CGACTACGAGGAATCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG3590.13170590328604395No Hit
CGACTACGAGGAATCCAGGGTTTTCCCAGTCACGACCTCCGCACAACAGT3570.13097216566328046No Hit
AAACTACGATAAGCCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT3540.12987155922913524No Hit
GTACTACGATAAGCCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT3520.12913782160637177No Hit
TTACTACGACTCAGCCAGGGTTTTCCCAGTCACGACCCCTGATGGCTACA3520.12913782160637177No Hit
GCACTACGATGCACCCAGGGTTTTCCCAGTCACGACTCTCACCTAAATCT3510.12877095279499004No Hit
AGACTACGATAAGCCCAGGGTTTTCCCAGTCACGACAAGACTGAAGGTCA3510.12877095279499004No Hit
AGACTACGATAAGCCCAGGGTTTTCCCAGTCACGACCTCTGAGCTGAATG3510.12877095279499004No Hit
GTACTACGATGCACCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT3480.12767034636084482No Hit
GGACTACGAGGAATCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG3460.12693660873808135No Hit
TGACTACGATAAGCCCAGGGTTTTCCCAGTCACGACCCTTCAATCTGAGG3440.1262028711153179No Hit
CAACTACGAGGAATCCAGGGTTTTCCCAGTCACGACCTGTGAACTTCCAG3430.12583600230393613No Hit
GAACTACGACTCAGCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG3430.12583600230393613No Hit
ATACTACGACTCAGCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG3430.12583600230393613No Hit
TTATCCTGATAAGCCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG3420.1254691334925544No Hit
GAACTACGACTCAGCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT3370.1236347894356457No Hit
AAACTACGACTCAGCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT3350.12290105181288224No Hit
GCACTACGAGGAATCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA3320.12180044537873702No Hit
CGACTACGACTCAGCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG3310.12143357656735528No Hit
TAACTACGATAAGCCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT3310.12143357656735528No Hit
TTATCCTGATAAGCCCAGGGTTTTCCCAGTCACGACCACAATCTCCTTCA3310.12143357656735528No Hit
TCACTACGACTCAGCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG3300.12106670775597354No Hit
TAACTACGACTCAGCCAGGGTTTTCCCAGTCACGACCCCTGATGGCTACA3270.11996610132182833No Hit
ATACTACGACTCAGCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT3260.11959923251044657No Hit
GTACTACGATGCACCCAGGGTTTTCCCAGTCACGACCCCTGATGGCTACA3250.11923236369906486No Hit
GTACTACGATGCACCCAGGGTTTTCCCAGTCACGACTGGCTACAATGTCT3250.11923236369906486No Hit
TTACTACGAGGAATCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT3220.11813175726491963No Hit
TTATCCTGATAAGCCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA3220.11813175726491963No Hit
AAACTACGACTCAGCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG3210.11776488845353791No Hit
CGACTACGACTCAGCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT3180.11666428201939269No Hit
CAACTACGAGGAATCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA3170.11629741320801094No Hit
CGACTACGATGCACCCAGGGTTTTCCCAGTCACGACTCTCACCTAAATCT3170.11629741320801094No Hit
GTACTACGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA3140.11519680677386573No Hit
TGACTACGATAAGCCCAGGGTTTTCCCAGTCACGACTACAGCAACTCTGG3130.114829937962484No Hit
GTACTACGATGCACCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT3120.11446306915110224No Hit
TGACTACGAGGAATCCAGGGTTTTCCCAGTCACGACGCTCTGAGATGAAT3100.11372933152833879No Hit
AAACTACGAGGAATCCAGGGTTTTCCCAGTCACGACCTGTGAACTTCCAG3100.11372933152833879No Hit
GGACTACGATAAGCCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG3090.11336246271695703No Hit
GGACTACGATAAGCCCAGGGTTTTCCCAGTCACGACGCTCTGAGATGAAT3080.1129955939055753No Hit
CTACTACGACTCAGCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG3050.11189498747143009No Hit
GCACTACGAGGAATCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT3030.11116124984866661No Hit
CAACTACGACTCAGCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG3010.11042751222590313No Hit
AAACTACGACTCAGCCAGGGTTTTCCCAGTCACGACCCCTGATGGCTACA2990.10969377460313967No Hit
CAACTACGAGGAATCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT2990.10969377460313967No Hit
TGACTACGATGCACCCAGGGTTTTCCCAGTCACGACTCTCACCTAAATCT2990.10969377460313967No Hit
GTACTACGACTCAGCCAGGGTTTTCCCAGTCACGACCAGACCCCAGGACC2980.10932690579175791No Hit
TTATCCTGATAAGCCCAGGGTTTTCCCAGTCACGACCTGTGAACTTCCAG2960.10859316816899446No Hit
TCACTACGAGGAATCCAGGGTTTTCCCAGTCACGACCTGTGAACTTCCAG2940.10785943054623097No Hit
CGACTACGACTCAGCCAGGGTTTTCCCAGTCACGACCCCTGATGGCTACA2900.10639195530070403No Hit
AGACTACGAGGAATCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG2890.10602508648932228No Hit
TGACTACGACTCAGCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG2880.10565821767794055No Hit
GGACTACGATAAGCCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG2840.10419074243241358No Hit
GTACTACGACTCAGCCAGGGTTTTCCCAGTCACGACTGGCTACAATGTCT2830.10382387362103185No Hit
GCACTACGATGCACCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT2830.10382387362103185No Hit
GAACTACGACTCAGCCAGGGTTTTCCCAGTCACGACCCCTGATGGCTACA2830.10382387362103185No Hit
TTACTACGAGGAATCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA2820.10345700480965013No Hit
AGACTACGATAAGCCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG2810.10309013599826837No Hit
CGACTACGATGCACCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT2810.10309013599826837No Hit
TTACTACGACTCAGCCAGGGTTTTCCCAGTCACGACTGGCTACAATGTCT2800.10272326718688664No Hit
AGACTACGATAAGCCCAGGGTTTTCCCAGTCACGACGCTCTGAGATGAAT2790.1023563983755049No Hit
TCACTACGACTCAGCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT2790.1023563983755049No Hit
ATACTACGACTCAGCCAGGGTTTTCCCAGTCACGACCCCTGATGGCTACA2780.10198952956412316No Hit
TGATCCTGATAAGCCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG2770.10162266075274143No Hit
GAACTACGAGGAATCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG2760.1012557919413597No Hit
TCACTACGACTCAGCCAGGGTTTTCCCAGTCACGACCCCTGATGGCTACA2750.10088892312997795No Hit
ACACTACGACTCAGCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG2750.10088892312997795No Hit
CTACTACGAGGAATCCAGGGTTTTCCCAGTCACGACCTGTGAACTTCCAG2750.10088892312997795No Hit
TAACTACGACTCAGCCAGGGTTTTCCCAGTCACGACTGGCTACAATGTCT2740.10052205431859622No Hit
GGACTACGATAAGCCCAGGGTTTTCCCAGTCACGACGCCTGATGGATCAA2730.10015518550721446No Hit
CTACTACGACTCAGCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT2730.10015518550721446No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCATA100.0014755856245.00002245
AAACTAC10350.0245.000021
CGATAAG56800.0245.000027
AGTAAAG100.0014755856245.00002245
ACGATGC45850.0245.000026
CGATGCA45850.0245.000027
CAATCCT3350.0245.01
CCACTAC6600.0245.01
TAACTAC13200.0245.01
AACTACG44400.0245.02
ATGCACC63100.0245.09
CTACGAT102650.0245.04
AGATCCT2850.0245.01
TACTACG46250.0245.02
ACTACGA210450.0245.03
CACTACG38600.0244.999982
CTACTAC7700.0244.999981
GATAAGC81250.0244.849248
GATGCAC63200.0244.806188
TACGATA56850.0244.784535
Produced by FastQC (version 0.11.5)