FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005466382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005466382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379161
Sequences flagged as poor quality0
Sequence length251
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACACTGAGGAATCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT11500.3033012361503425No Hit
AGCACACGACGAGGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT11220.29591651040059497No Hit
GGACACTGAGGAATCCAGGGTTTTCCCAGTCACGACCTCAGTTTGGTATA11050.29143292690967687No Hit
GTACACTGAGGAATCCAGGGTTTTCCCAGTCACGACCTCAGTTTGGTATA10260.2705974506871751No Hit
GTACACTGAGGAATCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT10020.26426768575882015No Hit
CGCACACGACGAGGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT9730.25661921980372454No Hit
GGACACTGAGGAATCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT9210.2429047291256221No Hit
GGACACTGAGGAATCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG9080.23947610645609646No Hit
GACACACGACGAGGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT8810.23235512091169713No Hit
CGACACTGAGGAATCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT8440.22259673331381657No Hit
GGACACTGAGGAATCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG8350.22022307146568346No Hit
GTACACTGATAAGCCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT8120.21415704674267658No Hit
TGCACACGACGAGGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT8080.2131020859212841No Hit
AGACACTGATAAGCCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT8070.21283834571593596No Hit
AAACACTGATAAGCCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT7940.20940972304641037No Hit
GTACACTGAGGAATCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT7850.20703606119827725No Hit
GGCACACGACGAGGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT7830.206508580787581No Hit
ATACACTGATAAGCCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT7460.19675019318970038No Hit
TGACACTGATAAGCCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT7330.1933215705201748No Hit
GGACACTGACTCAGCCAGGGTTTTCCCAGTCACGACGACATTCGTTCAAA7170.18910172723460483No Hit
TGACACTGATGCACCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA7130.18804676641321233No Hit
GTACACTGAGGAATCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG7120.1877830262078642No Hit
GAACACTGATAAGCCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT7060.18620058497577546No Hit
TTACACTGATAAGCCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT7050.18593684477042732No Hit
GGACACTGATGCACCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA7040.1856731045650792No Hit
ATCACACGACGAGGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT7020.18514562415438296No Hit
CGACACTGATGCACCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA6910.18224448189555362No Hit
CGACACTGAGGAATCCAGGGTTTTCCCAGTCACGACCTCAGTTTGGTATA6780.178815859226028No Hit
GTACACTGAGGAATCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA6650.17538723655650237No Hit
GGACACTGAGGAATCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA6600.17406853552976176No Hit
GCACACTGAGGAATCCAGGGTTTTCCCAGTCACGACCTCAGTTTGGTATA6480.17090365306558428No Hit
TAACACTGATAAGCCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT6440.1698486922441918No Hit
GTACACTGAGGAATCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG6270.16536510875327368No Hit
GTACACTGATGCACCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA6240.16457388813722929No Hit
GCACACTGAGGAATCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT6140.16193648608374805No Hit
TGACACTGAGGAATCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT6130.16167274587839994No Hit
CGACACTGAGGAATCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT6130.16167274587839994No Hit
GTCACACGACGAGGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT6060.15982656444096308No Hit
CACACACGACGAGGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT6060.15982656444096308No Hit
CGACACTGAGGAATCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG5940.1566616819767856No Hit
CCCACACGACGAGGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT5890.15534298095004495No Hit
GGACACTGACTCAGCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCTCAA5830.15376053971795622No Hit
TGACACTGAGGAATCCAGGGTTTTCCCAGTCACGACCTCAGTTTGGTATA5790.15270557889656372No Hit
GGACACTGACTCAGCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT5730.151123137664475No Hit
AAACACTGACGAGGCCAGGGTTTTCCCAGTCACGACGGCAAGGCCAAAGA5650.14901321602169No Hit
GGACACTGATAAGCCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT5600.14769451499494937No Hit
GGACACTGATGCACCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT5590.14743077478960126No Hit
ACCACACGACGAGGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT5590.14743077478960126No Hit
AACACACGACGAGGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT5490.14479337273612No Hit
AGACACTGATGCACCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA5480.1445296325307719No Hit
TTCACACGACGAGGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT5420.14294719129868314No Hit
CGACACTGATGCACCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT5410.14268345109333502No Hit
GCCACACGACGAGGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT5400.14241971088798688No Hit
CGACACTGAGGAATCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG5390.14215597068263877No Hit
GGACACTGAGGAATCCAGGGTTTTCCCAGTCACGACGGAACGATTTTCTG5310.1400460490398538No Hit
TTACACTGATGCACCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA5220.13767238719172067No Hit
GTACACTGAGGAATCCAGGGTTTTCCCAGTCACGACGGAACGATTTTCTG5180.13661742637032817No Hit
CAACACTGATAAGCCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT5120.13503498513823944No Hit
TCCACACGACGAGGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT5080.1339800243168469No Hit
AGCACACGACGAGGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA5060.1334525439061507No Hit
CGACACTGATAAGCCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT5040.13292506349545444No Hit
TGACACTGAAGGAGCCAGGGTTTTCCCAGTCACGACCCTTCAATCTGAGG5040.13292506349545444No Hit
CGACACTGAAGGAGCCAGGGTTTTCCCAGTCACGACCCTTCAATCTGAGG4920.12976018103127696No Hit
TACACACGACGAGGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT4900.1292327006205807No Hit
AAACACTGACGAGGCCAGGGTTTTCCCAGTCACGACTACAGCAACTCTGG4890.12896896041523256No Hit
GTACACTGACTCAGCCAGGGTTTTCCCAGTCACGACGACATTCGTTCAAA4870.12844148000453634No Hit
GCACACTGAGGAATCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT4840.12765025938849198No Hit
TGACACTGAGGAATCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG4840.12765025938849198No Hit
AGCACACGACGAGGCCAGGGTTTTCCCAGTCACGACCTGTGAACTTCCAG4830.12738651918314384No Hit
GCACACTGAGGAATCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG4800.12659529856709945No Hit
GGACACTGAAGGAGCCAGGGTTTTCCCAGTCACGACCCTTCAATCTGAGG4780.12606781815640322No Hit
ATACACTGATGCACCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA4750.12527659754035886No Hit
ACACACTGATAAGCCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT4720.12448537692431447No Hit
CTCACACGACGAGGCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT4710.12422163671896634No Hit
TCACACTGAGGAATCCAGGGTTTTCCCAGTCACGACCTCAGTTTGGTATA4680.12343041610292198No Hit
ACACACTGATGCACCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA4650.12263919548687761No Hit
GGACACTGATGCACCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA4630.12211171507618135No Hit
GTACACTGATGCACCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT4620.12184797487083322No Hit
TGACACTGAGGAATCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT4620.12184797487083322No Hit
TTACACTGAAGGAGCCAGGGTTTTCCCAGTCACGACCCTTCAATCTGAGG4600.12132049446013699No Hit
ATACACTGACGAGGCCAGGGTTTTCCCAGTCACGACGGCAAGGCCAAAGA4590.12105675425478886No Hit
TGACACTGAAGGAGCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT4580.12079301404944075No Hit
CGCACACGACGAGGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA4540.11973805322804823No Hit
ATACACTGATAAGCCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4540.11973805322804823No Hit
AGACACTGACGAGGCCAGGGTTTTCCCAGTCACGACGGCAAGGCCAAAGA4540.11973805322804823No Hit
GTACACTGAAGGAGCCAGGGTTTTCCCAGTCACGACCCTTCAATCTGAGG4530.11947431302270012No Hit
CGACACTGACGAGGCCAGGGTTTTCCCAGTCACGACTACAGCAACTCTGG4530.11947431302270012No Hit
GTACACTGATAAGCCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4520.11921057281735199No Hit
GGACACTGACTCAGCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA4520.11921057281735199No Hit
AGCACACGATGTTGCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT4500.11868309240665575No Hit
CTACACTGATAAGCCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT4500.11868309240665575No Hit
CTACACTGATGCACCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA4490.11841935220130763No Hit
CGACACTGACGAGGCCAGGGTTTTCCCAGTCACGACGGCAAGGCCAAAGA4470.11789187179061138No Hit
TGACACTGATGCACCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT4420.11657317076387075No Hit
GCACACTGAGGAATCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG4410.11630943055852264No Hit
GCACACTGATGCACCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA4390.11578195014782638No Hit
AGACACTGAGGAATCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT4380.11551820994247827No Hit
CGACACTGAAGGAGCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT4350.11472698932643388No Hit
GGACACTGAAGGAGCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT4330.11419950891573764No Hit
TCACACTGATAAGCCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT4270.11261706768364889No Hit
GGACACTGACTCAGCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT4210.11103462645156016No Hit
AGACACTGAGGAATCCAGGGTTTTCCCAGTCACGACCTCAGTTTGGTATA4210.11103462645156016No Hit
CGCACACGATGTTGCCAGGGTTTTCCCAGTCACGACACGACTGTCGCTAC4210.11103462645156016No Hit
CGACACTGAGGAATCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA4200.11077088624621204No Hit
AGACACTGACGAGGCCAGGGTTTTCCCAGTCACGACTACAGCAACTCTGG4190.11050714604086392No Hit
CGCACACGATGTTGCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT4180.11024340583551577No Hit
TGACACTGAGGAATCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG4180.11024340583551577No Hit
AGACACTGATAAGCCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4150.1094521852194714No Hit
TGACACTGATAAGCCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4080.10760600378203454No Hit
TCACACTGAGGAATCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT4040.10655104296064205No Hit
GTACACTGAAGGAGCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT4020.10602356254994581No Hit
ATCACACGAAGGAGCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA3990.10523234193390142No Hit
CGCACACGACGAGGCCAGGGTTTTCCCAGTCACGACCTGTGAACTTCCAG3990.10523234193390142No Hit
TGCACACGACGAGGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA3990.10523234193390142No Hit
TGACACTGATGCACCCAGGGTTTTCCCAGTCACGACCCTTCAATCTGAGG3990.10523234193390142No Hit
CCACACTGAGGAATCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT3990.10523234193390142No Hit
GAACACTGATGCACCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA3980.1049686017285533No Hit
GACACACGACGAGGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA3970.10470486152320518No Hit
TTACACTGAAGGAGCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT3960.10444112131785706No Hit
AAACACTGATAAGCCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT3950.10417738111250893No Hit
CCACACTGAGGAATCCAGGGTTTTCCCAGTCACGACCTCAGTTTGGTATA3930.1036499007018127No Hit
GGACACTGATGCACCCAGGGTTTTCCCAGTCACGACCCTTCAATCTGAGG3930.1036499007018127No Hit
AAACACTGACGAGGCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT3930.1036499007018127No Hit
AGCACACGATGTTGCCAGGGTTTTCCCAGTCACGACACGACTGTCGCTAC3920.10338616049646457No Hit
TTACACTGATGCACCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT3890.10259493988042019No Hit
GCACACTGAAGGAGCCAGGGTTTTCCCAGTCACGACCCTTCAATCTGAGG3880.10233119967507207No Hit
GAACACTGATAAGCCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT3880.10233119967507207No Hit
TAACACTGACGAGGCCAGGGTTTTCCCAGTCACGACGGCAAGGCCAAAGA3880.10233119967507207No Hit
TGACACTGAAGGAGCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA3870.10206745946972394No Hit
GCACACTGAGGAATCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA3860.10180371926437583No Hit
AGACACTGATGCACCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT3860.10180371926437583No Hit
GAACACTGACTCAGCCAGGGTTTTCCCAGTCACGACGACATTCGTTCAAA3840.10127623885367958No Hit
CGACACTGATGCACCCAGGGTTTTCCCAGTCACGACCCTTCAATCTGAGG3820.10074875844298331No Hit
TCACACTGATGCACCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA3820.10074875844298331No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)