FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005466399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005466399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42738
Sequences flagged as poor quality0
Sequence length251
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG22665.3020730965417195No Hit
AGCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG12833.002012260751556No Hit
GGTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG11162.6112593008563807No Hit
CGTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG11092.594880434273948No Hit
TAGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA10772.5200056156113995No Hit
AGTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG10732.510646263278581No Hit
GGCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG10442.4427909588656465No Hit
CGCCAACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA8672.028639618138425No Hit
TCTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG8191.916327390144602No Hit
CGTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA7831.8320932191492347No Hit
CGGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA6881.6098086012447939No Hit
TATTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG6671.5606720014974964No Hit
GCGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA6551.5325939444990406No Hit
AAGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA6211.453039449670083No Hit
TGGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG5961.3945434975899669No Hit
CGGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG5821.3617857644251017No Hit
TGGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG5641.3196686789274181No Hit
CGGCTAGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG5581.3056296504281903No Hit
TTTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG5511.2892507838457579No Hit
TGGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA5371.256493050680893No Hit
ACTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG5031.1769385558519352No Hit
CATTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG4711.1020637371893864No Hit
GTTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG4671.0927043848565678No Hit
TGTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA4451.041227947026066No Hit
GTGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG4150.9710328045299265No Hit
GGGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA4130.9663531283635172No Hit
GCCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG4020.9406149094482662No Hit
CGGAATGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA3860.9031775001169919No Hit
GCGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3700.8657400907857176No Hit
GCTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3680.8610604146193083No Hit
CGGACGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3680.8610604146193083No Hit
GAGCTAGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3640.8517010622864897No Hit
AATTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3520.8236230052880341No Hit
TGCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3470.8119238148720108No Hit
TGGACGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3450.8072441387056015No Hit
TCGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3420.8002246244559876No Hit
AGGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3200.7487481866254855No Hit
GGGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3050.7136506153774159No Hit
GGGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3030.7089709392110066No Hit
TGGAATGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA3000.7019514249613927No Hit
TTGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2980.6972717487949834No Hit
AGGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2890.6762132060461415No Hit
ACGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2870.6715335298797322No Hit
TGGCTAGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2830.6621741775469138No Hit
GATTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2830.6621741775469138No Hit
GTGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2790.6528148252140952No Hit
TTCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2670.6247367682156395No Hit
GGTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA2520.5896391969675698No Hit
ATGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2410.5639009780523188No Hit
CGGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2370.5545416257195003No Hit
TTGCTAGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2230.5217838925546352No Hit
GTCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2230.5217838925546352No Hit
AAGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2210.5171042163882259No Hit
CTGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA2160.5054050259722027No Hit
AGGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA2110.49370583555617953No Hit
CTACCGCGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2080.48668632130656564No Hit
CTGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2010.4703074547241331No Hit
GTACCGCGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1990.4656277785577238No Hit
TAGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1940.45392858814170056No Hit
CTGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1880.4398895596424727No Hit
GCCCAACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1860.43520988347606343No Hit
CCTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1790.418831016893631No Hit
TACCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1790.418831016893631No Hit
GAGCTAGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1790.418831016893631No Hit
CTTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1730.4047919883944031No Hit
CTCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1730.4047919883944031No Hit
CAGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1720.40245215031119846No Hit
ATGACGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1710.4001123122279938No Hit
TTACCGCGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1690.3954326360615846No Hit
CCGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1680.3930927979783799No Hit
TTGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1680.3930927979783799No Hit
TCCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1680.3930927979783799No Hit
ATTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1650.386073283728766No Hit
CAGCTAGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1650.386073283728766No Hit
CTCCAACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1610.3767139313959474No Hit
AGTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1590.3720342552295381No Hit
CAACCGCGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1520.3556553886471056No Hit
ATCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1470.3439561982310824No Hit
GACCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1470.3439561982310824No Hit
ATGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1430.33459684589826383No Hit
TTGACGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1410.32991716973185453No Hit
ATTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1390.32523749356544523No Hit
TCGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1390.32523749356544523No Hit
TCGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1370.320557817399036No Hit
TAGCTAGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1350.3158781412326267No Hit
ATGCTAGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1330.3111984650662174No Hit
AGGAATGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1300.3041789508166035No Hit
AGCCAACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1300.3041789508166035No Hit
AGGACGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1270.29715943656698957No Hit
CACCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1240.2901399223173756No Hit
AACCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1240.2901399223173756No Hit
GACCAACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1230.28780008423417097No Hit
TTTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1230.28780008423417097No Hit
TTGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1220.2854602461509664No Hit
CGCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1210.28312040806776173No Hit
TCGCTAGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1210.28312040806776173No Hit
CTGCTAGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1200.2807805699845571No Hit
GAACCGCGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1190.27844073190135243No Hit
CGGCTAGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1180.2761008938181478No Hit
ACGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1140.2667415414853292No Hit
CCGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1130.26440170340212454No Hit
ATCCAACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1110.2597220272357153No Hit
GAGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1070.2503626749028967No Hit
ATGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1070.2503626749028967No Hit
CAGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1060.2480228368196921No Hit
CTTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1060.2480228368196921No Hit
ATACCGCGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1040.24334316065328282No Hit
GTGCTAGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1030.24100332257007817No Hit
GTGACGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1000.23398380832046423No Hit
ACCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG960.22462445598764566No Hit
TGACCGCGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG940.21994477982123636No Hit
ACGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA920.21526510365482707No Hit
CAGCTAGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA910.21292526557162242No Hit
AGGCTAGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG910.21292526557162242No Hit
GAGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG890.20824558940521318No Hit
CCCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG880.20590575132200853No Hit
GCGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG860.20122607515559923No Hit
CACCAACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA860.20122607515559923No Hit
TAGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG850.1988862370723946No Hit
GGCCAACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA830.19420656090598531No Hit
GTGACGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG830.19420656090598531No Hit
TAACCGCGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG830.19420656090598531No Hit
GAGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG810.18952688473957602No Hit
GTTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA800.18718704665637137No Hit
CGGACGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA790.18484720857316672No Hit
GGGCTAGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG790.18484720857316672No Hit
ACGCTAGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG760.1778276943235528No Hit
GGGAATGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA760.1778276943235528No Hit
TCACCGCGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG760.1778276943235528No Hit
GTGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA730.1708081800739389No Hit
GCTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA730.1708081800739389No Hit
CGACCGCGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG710.1661285039075296No Hit
GCGCTAGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG690.16144882774112032No Hit
AAGAATGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA660.1544293134915064No Hit
CCCCAACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA640.1497496373250971No Hit
AAGCTAGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG640.1497496373250971No Hit
AAGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG620.1450699611586878No Hit
CATTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA610.1427301230754832No Hit
TGCCAACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA610.1427301230754832No Hit
CTGAATGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA610.1427301230754832No Hit
GGGACGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG590.1380504469090739No Hit
GCACCGCGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG590.1380504469090739No Hit
AGACCGCGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG590.1380504469090739No Hit
TAGAATGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA570.1333707707426646No Hit
GTGCTAGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA560.13103093265945998No Hit
CTGACGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG550.12869109457625533No Hit
TCGACGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG540.12635125649305068No Hit
ACACCGCGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG540.12635125649305068No Hit
TCGAATGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA520.12167158032664141No Hit
TGGCTAGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA510.11933174224343676No Hit
CAGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG510.11933174224343676No Hit
GGGCTAGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA510.11933174224343676No Hit
TTGCTAGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA510.11933174224343676No Hit
GCGCTAGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA490.11465206607702746No Hit
AAACCGCGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG490.11465206607702746No Hit
GGACCGCGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG470.10997238991061818No Hit
CCACCGCGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG460.10763255182741353No Hit
CCGCTAGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG440.10295287566100426No Hit
TAGCTAGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA440.10295287566100426No Hit
CTGCTAGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA430.10061303757779962No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)